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Ifih1 (基因名), Interferon-induced helicase C domain-containing protein 1 (蛋白名), ifih1_mouse.

产品名称:

Mouse Ifih1/ Interferon-induced helicase C domain-containing protein 1 ELISA Kit
干扰素诱导解旋酶C域蛋白1

货号:

E2803m

商标:

EIAab^®

监管等级:

RUO

别名:

Helicase with 2 CARD domains, Interferon induced with helicase C domain protein 1, Melanoma differentiation-associated protein 5, RIG-I-like receptor 2, Helicard, MDA-5, RLR-2

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

31.2-2000pg/mL

灵敏度:

15.65pg/mL

特异性:

Natural and recombinant mouse Interferon-induced helicase C domain-containing protein 1

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Immunology

规格 & 价格:

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Mouse Ifih1 ELISA Kit

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产品说明书

数据表:

说明书:

MSDS:

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数据
文献
序列 / 3D
常见问题解答
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通用注释

亚单元:

Monomer in the absence of ligands and homodimerizes in the presence of dsRNA ligands. Can assemble into helical or linear polymeric filaments on long dsRNA. Interacts with MAVS/IPS1. Interacts with V protein of Sendai virus. Binding to paramyxoviruses V proteins prevents IFN-beta induction, and the further establishment of an antiviral state. Interacts with PCBP2. Interacts with NLRC5. Interacts with PIAS2-beta. Interacts with DDX60. Interacts with ANKRD17. Interacts with IKBKE. Interacts (via the CARD domains) with TKFC, the interaction is inhibited by viral infection.

功能:

Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and theiler's murine encephalomyelitis virus (TMEV). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.

亚细胞位置:

Cytoplasm Nucleus May be found in the nucleus, during apoptosis.

数据库链接

UniGene:

Mm.136224

SMR:

Q8R5F7

STRING:

10090.ENSMUSP00000028259

KEGG:

mmu:71586

Pfam:

PF16739

Uniprot:

Q8R5F7

相关产品

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Human IFIH1 CLIA Kit

Human IFIH1 Protein

Human IFIH1 Polyclonal Antibody

Human IFIH1 Monoclonal Antibody

序列长度:

1025

序列:

仅供参考，详细请咨询（services@eiaab.com）

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1025:

KKWVELPIRF | PDLDYSEYCL | YSDED

3D 模型:

3D 结构:

免疫原序列:

验证序列:

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[1].

小鼠干扰素诱导解旋酶C域蛋白1(Ifih1)ELISA试剂盒可以做多少个样本？

小鼠干扰素诱导解旋酶C域蛋白1(Ifih1)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

小鼠干扰素诱导解旋酶C域蛋白1(Ifih1)ELISA试剂盒使用视频？

小鼠干扰素诱导解旋酶C域蛋白1(Ifih1)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

小鼠干扰素诱导解旋酶C域蛋白1(Ifih1)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的小鼠干扰素诱导解旋酶C域蛋白1(Ifih1)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

小鼠干扰素诱导解旋酶C域蛋白1(Ifih1)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

小鼠干扰素诱导解旋酶C域蛋白1(Ifih1)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析小鼠干扰素诱导解旋酶C域蛋白1(Ifih1)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

小鼠干扰素诱导解旋酶C域蛋白1(Ifih1)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集小鼠干扰素诱导解旋酶C域蛋白1(Ifih1)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

小鼠干扰素诱导解旋酶C域蛋白1(Ifih1)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

小鼠干扰素诱导解旋酶C域蛋白1(Ifih1)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

小鼠干扰素诱导解旋酶C域蛋白1(Ifih1)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Mouse Ifih1/ Interferon-induced helicase C domain-containing protein 1 ELISA Kit 干扰素诱导解旋酶C域蛋白1

E2803m

Helicase with 2 CARD domains, Interferon induced with helicase C domain protein 1, Melanoma differentiation-associated protein 5, RIG-I-like receptor 2, Helicard, MDA-5, RLR-2

Mouse Ifih1/ Interferon-induced helicase C domain-containing protein 1 ELISA Kit
干扰素诱导解旋酶C域蛋白1