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C1qbp (基因名), Complement component 1 Q subcomponent-binding protein, mitochondrial (蛋白名), c1qbp_rat.

产品名称:

Rat C1qbp/ Complement component 1 Q subcomponent-binding protein, mitochondrial ELISA Kit
补体成分1 Q子成分结合蛋白，线粒体

货号:

E8898r

商标:

EIAab^®

监管等级:

RUO

别名:

GC1q-R protein, Glycoprotein gC1qBP, C1qBP, Gc1qbp

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

1.56-100ng/mL

灵敏度:

0.87ng/mL

特异性:

Natural and recombinant rat Complement component 1 Q subcomponent-binding protein, mitochondrial

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Immunology

规格 & 价格:

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤5.1%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤8.3%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	84	80-90
Plasma	86	80-92

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	106-115%	112-121%	93-105%	96-108%
EDTA plasma(n=5)	109-119%	107-119%	105-117%	108-118%
heparin plasma(n=5)	102-112%	104-116%	96-106%	107-116%

通用注释

亚单元:

Homotrimer; three monomers form a donut-shaped structure with an unusually asymmetric charge distribution on the surface. Interacts with CDK13, HRK, VTN, NFYB, ADRA1B, FOXC1, DDX21, DDX50, NCL, SRSF1, SRSF9 and CDKN2A. Interacts with CD93; the association may represent a cell surface C1q receptor. Interacts with KRT1; the association represents a cell surface kininogen receptor. Interacts with CD209; the interaction is indicative for a C1q:C1QBP:CD209 signaling complex. Interacts with FBL and RRP1; the respective interactions with C1QBP are competetive. Probably associates with the mitoribosome. Interacts with MAVS; the interaction occurs upon viral transfection. Interacts with PPIF. Interacts with U2AF1L4.

功能:

Is believed to be a multifunctional and multicompartmental protein involved in inflammation and infection processes, ribosome biogenesis, regulation of apoptosis, transcriptional regulation and pre-mRNA splicing. At the cell surface is thought to act as an endothelial receptor for plasma proteins of the complement and kallikrein-kinin cascades. Putative receptor for C1q; specifically binds to the globular "heads" of C1q thus inhibiting C1; may perform the receptor function through a complex with C1qR/CD93. In complex with cytokeratin-1/KRT1 is a high affinity receptor for kininogen-1/HMWK. Can also bind other plasma proteins, such as coagulation factor XII leading to its autoactivation. May function to bind initially fluid kininogen-1 to the cell membrane. The secreted form may enhance both extrinsic and intrinsic coagulation pathways. It is postulated that the cell surface form requires docking with transmembrane proteins for downstream signaling which might be specific for a cell-type or response. By acting as C1q receptor is involved in chemotaxis of immature dendritic cells and neutrophils and is proposed to signal through CD209/DC-SIGN on immature dendritic cells, through integrin alpha-4/beta-1 during trophoblast invasion of the decidua, and through integrin beta-1 during endothelial cell adhesion and spreading. Signaling involved in inhibition of innate immune response is implicating the PI3K-AKT/PKB pathway. In mitochondrial translation may be involved in formation of functional 55S mitoribosomes; the function seems to involve its RNA-binding activity. May be involved in the nucleolar ribosome maturation process; the function may involve the exchange of FBL for RRP1 in the association with pre-ribosome particles. Involved in regulation of RNA splicing by inhibiting the RNA-binding capacity of SRSF1 and its phosphorylation. Is required for the nuclear translocation of splicing factor U2AF1L4. Involved in regulation of CDKN2A- and HRK-mediated apoptosis. May be involved in regulation of FOXC1 transcriptional activity and NFY/CCAAT-binding factor complex-mediated transcription. In infection processes acts as an attachment site for microbial proteins. May play a role in antibacterial defense. Involved in regulation of antiviral response by inhibiting DDX58- and IFIH1-mediated signaling pathways probably involving its association with MAVS after viral infection.

亚细胞位置:

Mitochondrion matrix Nucleus Cell membrane Peripheral membrane protein Extracellular side Secreted Cytoplasm Nucleus Nucleolus Seems to be predominantly localized to mitochondria.

数据库链接

UniGene:

Rn.2765

SMR:

O35796

STRING:

10116.ENSRNOP00000031020

KEGG:

rno:29681

Pfam:

PF02330

Uniprot:

O35796

相关产品

Human C1QBP ELISA Kit

Mouse C1qbp ELISA Kit

Bovine C1QBP ELISA Kit

Human C1QBP CLIA Kit

Mouse C1qbp CLIA Kit

序列长度:

279

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

279:

DELVELSTAL | EHQEYITFLE | DLKSFVKSQ

3D 模型:

3D 结构:

免疫原序列:

验证序列:

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[1].

大鼠补体成分1 Q子成分结合蛋白，线粒体(C1qbp)ELISA试剂盒可以做多少个样本？

大鼠补体成分1 Q子成分结合蛋白，线粒体(C1qbp)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

大鼠补体成分1 Q子成分结合蛋白，线粒体(C1qbp)ELISA试剂盒使用视频？

大鼠补体成分1 Q子成分结合蛋白，线粒体(C1qbp)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

大鼠补体成分1 Q子成分结合蛋白，线粒体(C1qbp)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的大鼠补体成分1 Q子成分结合蛋白，线粒体(C1qbp)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

大鼠补体成分1 Q子成分结合蛋白，线粒体(C1qbp)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

大鼠补体成分1 Q子成分结合蛋白，线粒体(C1qbp)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析大鼠补体成分1 Q子成分结合蛋白，线粒体(C1qbp)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

大鼠补体成分1 Q子成分结合蛋白，线粒体(C1qbp)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集大鼠补体成分1 Q子成分结合蛋白，线粒体(C1qbp)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

大鼠补体成分1 Q子成分结合蛋白，线粒体(C1qbp)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

大鼠补体成分1 Q子成分结合蛋白，线粒体(C1qbp)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

大鼠补体成分1 Q子成分结合蛋白，线粒体(C1qbp)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Rat C1qbp/ Complement component 1 Q subcomponent-binding protein, mitochondrial ELISA Kit 补体成分1 Q子成分结合蛋白，线粒体

E8898r

GC1q-R protein, Glycoprotein gC1qBP, C1qBP, Gc1qbp

Rat C1qbp/ Complement component 1 Q subcomponent-binding protein, mitochondrial ELISA Kit
补体成分1 Q子成分结合蛋白，线粒体