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Birc2 (基因名), Baculoviral IAP repeat-containing protein 2 (蛋白名), birc2_mouse.
产品名称:

Mouse Birc2/ Baculoviral IAP repeat-containing protein 2 ELISA Kit
重复序列包含蛋白2

货号:

E10854m

商标:
EIAab®
监管等级:
别名:

Cellular inhibitor of apoptosis 1, Inhibitor of apoptosis protein 2, RING-type E3 ubiquitin transferase BIRC2, C-IAP1, mIAP2

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.156-10ng/mL
灵敏度:
0.086ng/mL
特异性:
Natural and recombinant mouse Baculoviral IAP repeat-containing protein 2
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Cancer
Mouse Birc2 ELISA Kit
规格 & 价格: cart
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Mouse Birc2 ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS
在线询价


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤8.3%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤10.1%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

95

89-101

Plasma

97

91-103

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

97-107%

80-88%

93-102%

97-107%

EDTA plasma(n=5)

84-84%

106-116%

109-119%

95-107%

heparin plasma(n=5)

109-119%

 

91-100%

108-118%

100-109%

 

通用注释


亚单元:
Interacts with DIABLO/SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. Interacts with CASP9. Interacts (via BIR domains) with TRAF2; the interaction is required for IKBKE ubiquitination. Interacts with E2F1, RIPK1, RIPK2, RIPK3, RIPK4, BIRC5/survivin and USP19 (By similarity). Interacts with HSP90AB1.


功能:
Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO, IKBKE and MXD1/MAD1. Can also function as an E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle.


亚细胞位置:
Cytoplasm Nucleus Agents that induce either the extrinsic or intrinsic apoptotic pathways promote its redistribution from the nuclear compartment to the cytoplasmic compartment. Associated with the midbody in telophase cells, and found diffusely in the nucleus of interphase cells (By similarity).


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