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AKT1 (基因名), RAC-alpha serine/threonine-protein kinase (蛋白名), akt1_bovin.

产品名称:

Bovine AKT1/ RAC-alpha serine/threonine-protein kinase ELISA Kit
α-丝氨酸/苏氨酸蛋白激酶

货号:

E0382b

商标:

EIAab^®

监管等级:

RUO

别名:

Protein kinase B, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB alpha, PKB

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.312-20ng/mL

灵敏度:

0.099ng/mL

特异性:

Natural and recombinant bovine RAC-alpha serine/threonine-protein kinase

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Cancer

规格 & 价格:

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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通用注释

亚单元:

Interacts (via the C-terminus) with CCDC88A (via its C-terminus) and THEM4 (via its C-terminus). Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with TRAF6. Interacts with GRB10; the interaction leads to GRB10 phosphorylation thus promoting YWHAE binding. Interacts with RARA; the interaction phosphorylates RARA and represses its transactivation activity. Interacts with MAP3K5 and TNK2. Interacts with BAD, CLK2, PPP2R5B, STK3 and STK4. Interacts (via PH domain) with SIRT1. Interacts with SRPK2 in a phosphorylation-dependent manner. Interacts with RAF1. Interacts with PKN2 (via C-terminal domain); the interaction occurs with the C-terminus cleavage products of PKN2 in apoptotic cells. Interacts with TRIM13; the interaction ubiquitinates AKT1 leading to its proteasomal degradation. Interacts with and phosphorylated by PDPK1. Interacts with BTBD10. Interacts with KCTD20. Interacts with PA2G4. Interacts with PA2G4. Interacts with KIF14; the interaction is detected in the plasma membrane upon INS stimulation and promotes AKT1 phosphorylation. Interacts with FAM83B; activates the PI3K/AKT signaling cascade. Interacts with WDFY2 (via WD repeats 1-3). Forms a complex with WDFY2 and FOXO1. Interacts with FAM168A.

功能:

AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation.

亚细胞位置:

Cytoplasm Nucleus Cell membrane Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A (By similarity). Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles (By similarity).

数据库链接

UniGene:

Bt.4843

SMR:

Q01314

KEGG:

bta:280991

Pfam:

PF00169

Uniprot:

Q01314

相关产品

序列长度:

480

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

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480:

PPDQDDSMEG | VDSERRPHFP | QFSYSASATA |

3D 模型:

3D 结构:

免疫原序列:

验证序列:

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[1].

牛α-丝氨酸/苏氨酸蛋白激酶(AKT1)ELISA试剂盒可以做多少个样本？

牛α-丝氨酸/苏氨酸蛋白激酶(AKT1)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

牛α-丝氨酸/苏氨酸蛋白激酶(AKT1)ELISA试剂盒使用视频？

牛α-丝氨酸/苏氨酸蛋白激酶(AKT1)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

牛α-丝氨酸/苏氨酸蛋白激酶(AKT1)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的牛α-丝氨酸/苏氨酸蛋白激酶(AKT1)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

牛α-丝氨酸/苏氨酸蛋白激酶(AKT1)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

牛α-丝氨酸/苏氨酸蛋白激酶(AKT1)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析牛α-丝氨酸/苏氨酸蛋白激酶(AKT1)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

牛α-丝氨酸/苏氨酸蛋白激酶(AKT1)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集牛α-丝氨酸/苏氨酸蛋白激酶(AKT1)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

牛α-丝氨酸/苏氨酸蛋白激酶(AKT1)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

牛α-丝氨酸/苏氨酸蛋白激酶(AKT1)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

牛α-丝氨酸/苏氨酸蛋白激酶(AKT1)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Bovine AKT1/ RAC-alpha serine/threonine-protein kinase ELISA Kit α-丝氨酸/苏氨酸蛋白激酶

E0382b

Protein kinase B, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB alpha, PKB

Bovine AKT1/ RAC-alpha serine/threonine-protein kinase ELISA Kit
α-丝氨酸/苏氨酸蛋白激酶