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OPTN (基因名), Optineurin (蛋白名), OPTN_HUMAN.

产品名称:

Human OPTN/ Optineurin ELISA Kit
视神经素

货号:

E15443h

商标:

EIAab^®

监管等级:

RUO

别名:

E3-14.7K-interacting protein, FIP-2, Huntingtin yeast partner L, Huntingtin-interacting protein 7, Huntingtin-interacting protein L, NEMO-related protein, Optic neuropathy-inducing protein, Transcription factor IIIA-interacting protein, HIP-7, TFIIIA-IntP, FIP2, GLC1E, HIP7, HYPL, NRP

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.312-20ng/mL

灵敏度:

0.163ng/mL

特异性:

Natural and recombinant human Optineurin

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Immunology

规格 & 价格:

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Human OPTN ELISA Kit

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产品说明书

数据表:

说明书:

MSDS:

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数据
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序列 / 3D
常见问题解答
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通用注释

亚单元:

Interacts with HD, Rab8 (RAB8A and/or RAB8B) (active GTP-bound form), GTF3A, TRAF3, TBK1, MYO6 and TFRC. Binds to linear ubiquitin chains. Interacts with LC3 family members MAP1LC3A, MAP1LC3B, GABARAP, GABARAPL1 and GABARAPL2; OPTN phosphorylation increases the association (at least with MAP1LC3B). Self-associates. Interacts with RAB12; the interaction may be indirect. Interacts with E3 14.7 kDa protein of group C human adenovirus.

功能:

Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection. Plays a neuroprotective role in the eye and optic nerve. Probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as of transferrin receptor (TFRC/TfR); regulates Rab8 recruitnment to tubules emanating from the endocytic recycling compartment. Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family; targets ubiquitin-coated bacteria (xenophagy), such as cytoplasmic Salmonella enterica, and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52. May constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death.

亚细胞位置:

Cytoplasm Perinuclear region Golgi apparatus Golgi apparatus Trans-Golgi network Cytoplasmic vesicle Autophagosome Cytoplasmic vesicle Recycling endosome Found in the perinuclear region and associates with the Golgi apparatus. Colocalizes with MYO6 and RAB8 at the Golgi complex and in vesicular structures close to the plasma membrane. Localizes to LC3-positive cytoplasmic vesicles upon induction of autophagy.

数据库链接

UniGene:

Hs.332706

SMR:

Q96CV9

STRING:

9606.ENSP00000263036

KEGG:

hsa:10133

MIM:

137760

Pfam:

PF16516

Uniprot:

Q96CV9

相关产品

Mouse Optn ELISA Kit

Rat Optn ELISA Kit

Chicken OPTN ELISA Kit

Pig OPTN ELISA Kit

Mouse Optn CLIA Kit

序列长度:

577

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

577:

PIHSCPKCGE | VLPDIDTLQI | HVMDCII

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

人视神经素(OPTN)ELISA试剂盒可以做多少个样本？

人视神经素(OPTN)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人视神经素(OPTN)ELISA试剂盒使用视频？

人视神经素(OPTN)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人视神经素(OPTN)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人视神经素(OPTN)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人视神经素(OPTN)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人视神经素(OPTN)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人视神经素(OPTN)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人视神经素(OPTN)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人视神经素(OPTN)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人视神经素(OPTN)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人视神经素(OPTN)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人视神经素(OPTN)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Human OPTN/ Optineurin ELISA Kit 视神经素

E15443h

E3-14.7K-interacting protein, FIP-2, Huntingtin yeast partner L, Huntingtin-interacting protein 7, Huntingtin-interacting protein L, NEMO-related protein, Optic neuropathy-inducing protein, Transcription factor IIIA-interacting protein, HIP-7, TFIIIA-IntP, FIP2, GLC1E, HIP7, HYPL, NRP

Human OPTN/ Optineurin ELISA Kit
视神经素