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DEFB126 (基因名), Beta-defensin 126 (蛋白名), DB126_HUMAN.
产品名称:

Human DEFB126/ Beta-defensin 126 ELISA Kit
Beta-defensin 126

货号:

E2040h

商标:
EIAab®
监管等级:
别名:

Beta-defensin 26, Defensin, beta 126, Epididymal secretory protein 13.2, HBD26, DEFB-26, ESP13.2, C20orf8, DEFB26

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.625-40ng/ml
灵敏度:
0.156ng/mL
特异性:
Natural and recombinant human Beta-defensin 126
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
-
Human DEFB126 ELISA Kit
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产品说明书
说明书: 下载说明书
MSDS: MSDS
在线询价


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤5.3%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤9.1%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

101

95-107

Plasma

103

97-109

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

105-115%

110-120%

106-115%

94-107%

EDTA plasma(n=5)

97-107%

80-90%

93-102%

110-120%

heparin plasma(n=5)

110-120%

 

108-118%

101-111%

93-102%

 

通用注释


亚单元:
Homodimer or homooligomer; disulfide-linked.


功能:
Highly glycosylated atypical beta-defensin involved in several aspects of sperm function. Facilitates sperm transport in the female reproductive tract and contributes to sperm protection against immunodetection; both functions are probably implicating the negative surface charge provided by its O-linked oligosaccharides in the sperm glycocalyx. Involved in binding of sperm to oviductal epithelial cells to form a sperm reservoir until ovulation. Release from the sperm surface during capacitation and ovaluation by an elevation of oviductal fluid pH is unmasking other surface components and allows sperm to penetrate the cumulus matrix and bind to the zona pellucida of the oocyte (By similarity). In vitro has antimicrobial activity and may inhibit LPS-mediated inflammation (PubMed:19373462, PubMed:23229569).


亚细胞位置:
Secreted Secreted by epididymal cells and is absorbed to the surface of sperm during transit through the epididymis (By similarity). Mainly located on the sperm acrosome.


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