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Arc (基因名), Activity-regulated cytoskeleton-associated protein (蛋白名), ARC_MOUSE.

产品名称:

Mouse Arc/ Activity-regulated cytoskeleton-associated protein ELISA Kit
细胞骨架活性调节蛋白

货号:

E7736m

商标:

EIAab^®

监管等级:

RUO

别名:

Activity-regulated gene 3.1 protein, ARC/ARG3.1, mArc

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.156-10ng/mL

灵敏度:

0.087ng/mL

特异性:

Natural and recombinant mouse Activity-regulated cytoskeleton-associated protein

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

规格 & 价格:

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产品说明书

数据表:

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤6.4%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤9.0%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	83	80-89
Plasma	85	80-91

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	112-122%	107-119%	87-96%	105-114%
EDTA plasma(n=5)	80-91%	100-111%	91-103%	101-110%
heparin plasma(n=5)	93-103%	98-109%	109-119%	93-105%

通用注释

亚单元:

Homooligomer; homooligomerizes into virion-like capsids (PubMed:31151856). Interacts with SH3GL1/endophilin-2, SH3GL3/endophilin-3 and DNM2/DYN2 (By similarity). Interacts with CAMK2B (in the kinase inactive state); leading to target ARC to inactive synapses (By similarity). Interacts with PSEN1 (PubMed:22036569). Interacts with GRIN2A and GRIN2B; inhibiting homooligomerization (By similarity).

功能:

Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system (By similarity). ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation (By similarity). ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons (By similarity). Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory (PubMed:29264923, PubMed:24094104, PubMed:31151856). Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis (PubMed:17088213, PubMed:20211139, PubMed:20228806). Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses (PubMed:23791196). Accumulates at weaker synapses, probably to prevent their undesired enhancement (By similarity). This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material (By similarity). Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory (PubMed:17088210, PubMed:20228806). Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1 (PubMed:22036569). In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation (PubMed:28783680).

亚细胞位置:

Extracellular vesicle membrane Lipid-anchor Cell junction Synapse Postsynaptic cell membrane Lipid-anchor Cell junction Synapse Cell junction Synapse Postsynaptic cell membrane Postsynaptic density Early endosome membrane Cell projection Dendrite Cytoplasm Cytoskeleton Cytoplasm Cell cortex Cell projection Dendritic spine Cytoplasmic vesicle Secretory vesicle Acrosome Forms virion-like extracellular vesicles that are released from neurons (By similarity). Enriched in postsynaptic density of dendritic spines (By similarity). Targeted to inactive synapses following interaction with CAMK2B in the kinase inactive state (By similarity). Accumulation at weaker synapses may be required to prevent their undesired enhancement (By similarity). Associated with the cell cortex of neuronal soma and dendrites (By similarity). Associated with the sperm tail (PubMed:12493697).

数据库链接

UniGene:

Mm.491310

SMR:

Q9WV31

STRING:

10090.ENSMUSP00000023268

KEGG:

mmu:11838

Uniprot:

Q9WV31

相关产品

Human ARC ELISA Kit

Rat Arc ELISA Kit

Chicken ARC ELISA Kit

Human ARC CLIA Kit

Rat Arc CLIA Kit

序列长度:

396

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

402:

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

小鼠细胞骨架活性调节蛋白(Arc)ELISA试剂盒可以做多少个样本？

小鼠细胞骨架活性调节蛋白(Arc)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

小鼠细胞骨架活性调节蛋白(Arc)ELISA试剂盒使用视频？

小鼠细胞骨架活性调节蛋白(Arc)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

小鼠细胞骨架活性调节蛋白(Arc)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的小鼠细胞骨架活性调节蛋白(Arc)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

小鼠细胞骨架活性调节蛋白(Arc)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

小鼠细胞骨架活性调节蛋白(Arc)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析小鼠细胞骨架活性调节蛋白(Arc)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

小鼠细胞骨架活性调节蛋白(Arc)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集小鼠细胞骨架活性调节蛋白(Arc)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

小鼠细胞骨架活性调节蛋白(Arc)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

小鼠细胞骨架活性调节蛋白(Arc)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

小鼠细胞骨架活性调节蛋白(Arc)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Mouse Arc/ Activity-regulated cytoskeleton-associated protein ELISA Kit 细胞骨架活性调节蛋白

E7736m

Activity-regulated gene 3.1 protein, ARC/ARG3.1, mArc

Mouse Arc/ Activity-regulated cytoskeleton-associated protein ELISA Kit
细胞骨架活性调节蛋白