Hspb1 (基因名), Heat shock protein beta-1 (蛋白名), hspb1_rat.
产品名称:
Rat Hspb1/ Heat shock protein beta-1 CLIA Kit
热休克蛋白27
货号:
U0693r
商标:
EIAab®
监管等级:
别名:
Heat shock 27 kDa protein, HSP 27, HspB1, Hsp27
检测方法:
CLIA
实验类型:
Sandwich
检测范围:
39-2500pg/mL
特异性:
Natural and recombinant rat Heat shock protein beta-1
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
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研究领域:
Epigenetics
精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: 3.9%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: 7.7%
批内 CV: 3.9%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: 7.7%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
99 |
91-103 |
Plasma |
98 |
93-106 |
线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
92-105% |
90-101% |
95-105% |
92-109% |
EDTA plasma(n=5) |
89-95% |
93105% |
89-94% |
90-106% |
heparin plasma(n=5) |
93-104%
|
94-110% |
101-111% |
91-110% |
通用注释
亚单元:
Homooligomer. Homodimer; becomes monomeric upon activation. Heterooligomer; with HSPB6. Associates with alpha- and beta-tubulin (By similarity). Interacts with TGFB1I1 (PubMed:11546764). Interacts with CRYAB (By similarity). Interacts with HSPB8 (By similarity). Interacts with HSPBAP1 (PubMed:10751411).
功能:
Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.
亚细胞位置:
Cytoplasm
Nucleus
Cytoplasm
Cytoskeleton
Spindle
Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
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