首页 > 重组蛋白 > Human PLAT Recombinant Protein

PLAT (基因名), Tissue-type plasminogen activator (蛋白名), tpa_human.

产品名称:

Human PLAT/ Tissue-type plasminogen activator Recombinant Protein
组织型纤溶酶原激活剂

货号:

R0525h

商标:

EIAab^®

监管等级:

RUO

别名:

t-PA

序列号:

P00750

来源:

E.coli

种属:

Human

标签:

His

序列:

311-562aa

预估分子量:

27.72 kDa (monomer)

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

关注

Human PLAT Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献 ( 1 )
出版物
序列 / 3D
常见问题解答
反馈墙 (0)

R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 8p11.21 by HGNC 8p11.21 by Entrez Gene 8p11.21 by Ensembl

PLAT Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Heterodimer of chain A and chain B held by a disulfide bond. Forms a heterodimer with SERPINA5. Binds to fibrin with high affinity. This interaction leads to an increase in the catalytic efficiency of the enzyme between 100-fold and 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binds to annexin A2, cytokeratin-8, fibronectin and laminin. Binds to mannose receptor and the low-density lipoprotein receptor-related protein (LRP1); these proteins are involved in TPA clearance. Yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. In addition, binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold. Binds LRP1B; binding is followed by internalization and degradation.

功能:

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration.

亚细胞位置:

Secreted Extracellular space

数据库链接

UniGene:

Hs.491582

SMR:

P00750

STRING:

9606.ENSP00000220809

KEGG:

hsa:5327

MIM:

173370

Pfam:

PF00008

Uniprot:

P00750

相关产品

Mouse Plat ELISA Kit

Rat Plat ELISA Kit

Bovine PLAT ELISA Kit

Pig PLAT ELISA Kit

Mouse Plat CLIA Kit

序列长度:

562

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

562:

NYLDWIRDNM | RP

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Cocktail Blood Biomarkers: Prediction of Clinical Outcomes in Patients with Acute Ischemic Stroke"

Zeng L. · Liu J. · Wang Y. · Wang L. · Weng S. · Chen S. · Yang G.-Y.

From: European Neurology

[1].

"Male-female differences in the genetic regulation of t-PA and PAI-1 levels in a Ghanaian population."

(PMID: 18953568)

[2].

"Tissue plasminogen activator genetic polymorphisms do not influence tissue plasminogen activator release in patients with coronary heart disease."

(PMID: 16836660)

[3].

"Common genetic variation in five thrombosis genes and relations to plasma hemostatic protein level and cardiovascular disease risk."

(PMID: 16614319)

[4].

"Is tissue-plasminogen activator gene polymorphism a risk factor for venous thromboembolism in every population?"

(PMID: 15976969)

[5].

"Influence of the Alu-repeat I/D polymorphism in t-PA gene intron 8 on the stimulated t-PA release after venous occlusion."

(PMID: 12643326)

[6].

"Genetic variation at the human tissue-type plasminogen activator (tPA) locus: haplotypes and analysis of association to plasma levels of tPA."

(PMID: 12891381)

[7].

"Linkage disequilibrium and haplotype analysis among eight novel single-nucleotide polymorphisms in the human tissue-type plasminogen activator (t-PA) gene."

(PMID: 11450845)

[8].

"Lysine 156 promotes the anomalous proenzyme activity of tPA: X-ray crystal structure of single-chain human tPA."

(PMID: 9305622)

[9].

"The solution structure and backbone dynamics of the fibronectin type I and epidermal growth factor-like pair of modules of tissue-type plasminogen activator."

(PMID: 7582899)

[10].

"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

(PMID: 20628086)

[1].

人组织型纤溶酶原激活剂(PLAT)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人组织型纤溶酶原激活剂(PLAT)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人组织型纤溶酶原激活剂(PLAT)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人组织型纤溶酶原激活剂(PLAT)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人组织型纤溶酶原激活剂(PLAT)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人组织型纤溶酶原激活剂(PLAT)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人组织型纤溶酶原激活剂(PLAT)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人组织型纤溶酶原激活剂(PLAT)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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