首页 > 重组蛋白 > Human TERT Recombinant Protein

TERT (基因名), Telomerase reverse transcriptase (蛋白名), tert_human.

产品名称:

Human TERT/ Telomerase reverse transcriptase Recombinant Protein
端粒酶逆转录酶

货号:

R2133h

商标:

EIAab^®

监管等级:

RUO

别名:

HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2, EST2, TCS1, TRT

序列号:

O14746

来源:

E.coli

种属:

Human

标签:

His

序列:

376-521aa

预估分子量:

16.06 kDa (monomer)

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

规格 & 价格:

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Human TERT Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献 ( 1 )
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 5p15.33 by HGNC 5p15.33 by Entrez Gene 5p15.33 by Ensembl

TERT Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Homodimer; dimerization is required to produce a functional complex. Oligomer; can form oligomers in the absence of the telomerase RNA template component (TERC). Catalytic subunit of the telomerase holoenzyme complex composed minimally of TERT and TERC. The telomerase complex is composed of TERT, DKC1, WDR79/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and a telomerase RNA template component (TERC). The molecular chaperone HSP90/P23 complex is required for correct assembly and stabilization of the active telomerase. Interacts directly with HSP90A and PTGES3. Interacts with HSPA1A; the interaction occurs in the absence of TERC and dissociates once the complex has formed. Interacts with RAN; the interaction promotes nuclear export of TERT. Interacts with XPO1. Interacts with PTPN11; the interaction retains TERT in the nucleus. Interacts with NCL (via RRM1 and C-terminal RRM4/Arg/Gly-rich domains); the interaction is important for nucleolar localization of TERT. Interacts with SMARCA4 (via the bromodomain); the interaction regulates Wnt-mediated signaling. Interacts with MCRS1 (isoform MCRS2); the interaction inhibits in vitro telomerase activity. Interacts with PIF1; the interaction has no effect on the elongation activity of TERT. Interacts with PML; the interaction recruits TERT to PML bodies and inhibits telomerase activity. Interacts with GNL3L (By similarity). Interacts with isoform 1 and isoform 2 of NVL (PubMed:22226966).

功能:

Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.

亚细胞位置:

Nucleus Nucleolus Nucleus Nucleoplasm Nucleus Chromosome Telomere Cytoplasm Nucleus PML body Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage. Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress-mediated phosphorylation at Tyr-707. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT.

数据库链接

UniGene:

Hs.492203

SMR:

O14746

STRING:

9606.ENSP00000309572

KEGG:

hsa:7015

MIM:

178500

Pfam:

PF00078

Uniprot:

O14746

相关产品

Mouse Tert ELISA Kit

Rat Tert ELISA Kit

Bovine TERT ELISA Kit

Dog TERT ELISA Kit

Mouse Tert CLIA Kit

序列长度:

1132

序列:

仅供参考，详细请咨询（services@eiaab.com）

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1132:

TQLSRKLPGT | TLTALEAAAN | PALPSDFKTI | LD

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Differential decrease in soluble and DNA-bound telomerase in senescent human fibroblasts"

S Yehuda, H Yanai, E Priel, et al.

From: Biogerontology

[1].

"Constitutional hypomorphic telomerase mutations in patients with acute myeloid leukemia."

(PMID: 19147845)

[2].

"Mutations in TERT, the gene for telomerase reverse transcriptase, in aplastic anemia."

(PMID: 15814878)

[3].

"TERC and TERT gene mutations in patients with bone marrow failure and the significance of telomere length measurements."

(PMID: 18931339)

[4].

"PML-IV functions as a negative regulator of telomerase by interacting with TERT."

(PMID: 19567472)

[5].

"Nuclear protein tyrosine phosphatase Shp-2 is one important negative regulator of nuclear export of telomerase reverse transcriptase."

(PMID: 18829466)

[1].

人端粒酶逆转录酶(TERT)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人端粒酶逆转录酶(TERT)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人端粒酶逆转录酶(TERT)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人端粒酶逆转录酶(TERT)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人端粒酶逆转录酶(TERT)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人端粒酶逆转录酶(TERT)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人端粒酶逆转录酶(TERT)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人端粒酶逆转录酶(TERT)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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