首页 > 重组蛋白 > Human SPP1 Recombinant Protein

SPP1 (基因名), Osteopontin (蛋白名), ostp_human.

产品名称:

Human SPP1/ Osteopontin Recombinant Protein
骨桥素

货号:

R0899h

商标:

EIAab^®

监管等级:

RUO

别名:

Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, SPP-1, PSEC0156, BNSP, OPN

序列号:

P10451

来源:

E.coli

种属:

Human

标签:

His

序列:

24-130aa

预估分子量:

11.77 kDa (monomer)

纯度:

Greater than 95% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

规格 & 价格:

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Human SPP1 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献 ( 5 )
出版物
序列 / 3D
常见问题解答
反馈墙 (0)

R&D 技术数据

The PCR product of human SPP1 gene was determined by 1% Agarose stained with EB.

Recombinant human SPP1 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 4q22.1 by HGNC 4q22.1 by Entrez Gene 4q22.1 by Ensembl

SPP1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Ligand for integrin alpha-V/beta-3.

功能:

Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

亚细胞位置:

Secreted

数据库链接

UniGene:

Hs.313

STRING:

9606.ENSP00000378517

KEGG:

hsa:6696

MIM:

166490

Pfam:

PF00865

Uniprot:

P10451

相关产品

Mouse Spp1 ELISA Kit

Rat Spp1 ELISA Kit

Bovine SPP1 ELISA Kit

Chicken SPP1 ELISA Kit

Pig SPP1 ELISA Kit

序列长度:

314

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

314:

RISHELDSAS | SEVN

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"The Effect of Low-Magnitude Low-Frequency Vibrations (LMLF) on Osteogenic Differentiation Potential of Human Adipose Derived Mesenchymal Stem Cells"

Monika M, Daniel L, Krzysztof A.T, et al.

From: Cellular and Molecular Bioengineering

[2].

"Lithium ions (Li+) and nanohydroxyapatite (nHAp) doped with Li+ enhance expression of late osteogenic markers in adipose-derived stem cells. Potential theranostic application of nHAp doped with Li+ and co-doped with europium (III) and samarium (III) ions"

M. Alicka, P. Sobierajska, K. Kornicka, et al.

From: Materials Science and Engineering: C

[3].

"Elevated osteopontin level of synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis patients"

S. G. Gaoya, K. H. Liya, K. B. Zengyz, M. Tuyx, M. Xuy and G. H. Lei

From: Osteoarthritis and Cartilage

[4].

"SERUM OSTEOPONTIN LEVEL AS A TUMOR MARKER IN CIRRHOTIC PATIENTS WITH HEPATOCELLULAR CARCINOMA IN COMPARISON TO ALPHA-FETO PROTEIN"

MAA El-Moneim, KF Sayed, AFA Elaziz, et al.

From: Al Azhar Medical Journal

[5].

"Proliferation and osteogenesis of immortalized bone marrow-derived mesenchymal stem cells in porous polylactic glycolic acid scaffolds under..."

Yang J, Cao C, Wang W, et al.

From: Journal of biomedical materials research Part A

[1].

"The implication of osteopontin (OPN) expression and genetic polymorphisms of OPN promoter in oral carcinogenesis."

(PMID: 20219412)

[2].

"The impact of osteopontin promoter polymorphisms on the risk of calcium urolithiasis."

(PMID: 20144595)

[3].

"Association between osteopontin and human abdominal aortic aneurysm."

(PMID: 17170369)

[4].

"Post-translationally modified residues of native human osteopontin are located in clusters: identification of 36 phosphorylation and five O-glycosylation sites and their biological implications."

(PMID: 15869464)

[5].

"A polymorphism of the osteopontin gene is related to urinary calcium stones."

(PMID: 16145474)

[6].

"Overexpression of osteopontin in rheumatoid synovial mononuclear cells is associated with joint inflammation, not with genetic polymorphism."

(PMID: 15742430)

[7].

"Gene polymorphisms, bone mineral density and bone mineral content in young children: the Iowa Bone Development Study."

(PMID: 12879219)

[8].

"Expression of osteopontin in human glioma. Its correlation with the malignancy."

(PMID: 7837791)

[9].

"Molecular cloning and sequencing of cDNA encoding urinary stone protein, which is identical to osteopontin."

(PMID: 1575754)

[10].

"Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily."

(PMID: 1729712)

[1].

人骨桥素(SPP1)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人骨桥素(SPP1)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人骨桥素(SPP1)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人骨桥素(SPP1)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人骨桥素(SPP1)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人骨桥素(SPP1)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人骨桥素(SPP1)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人骨桥素(SPP1)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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