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ISG15 (基因名), Ubiquitin-like protein ISG15 (蛋白名), isg15_human.

产品名称:

Human ISG15/ Ubiquitin-like protein ISG15 CLIA Kit
泛素样蛋白ISG15

货号:

U14926h

商标:

EIAab^®

监管等级:

RUO

别名:

Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein, Ubiquitin cross-reactive protein, IP17, hUCRP, G1P2, UCRP

检测方法:

CLIA

实验类型:

Sandwich

检测范围:

3.9-250pg/mL

特异性:

Natural and recombinant human Ubiquitin-like protein ISG15

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

Immunology

规格 & 价格:

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产品说明书

说明书:

MSDS:

在线询价

数据
文献 ( 1 )
序列 / 3D
反馈墙 (0)

精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： 3.9%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： 7.7%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

通用注释

亚单元:

(Microbial infection) Interacts with vaccinia virus protein E3 (PubMed:24257616). Interaction with influenza B NS1 protein inhibits its conjugation (PubMed:11157743).

功能:

Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include IFIT1, MX1/MxA, PPM1B, UBE2L6, UBA7, CHMP5, CHMP2A, CHMP4B and CHMP6. Can also isgylate: EIF2AK2/PKR which results in its activation, DDX58/RIG-I which inhibits its function in antiviral signaling response, EIF4E2 which enhances its cap structure-binding activity and translation-inhibition activity, UBE2N and UBE2E1 which negatively regulates their activity, IRF3 which inhibits its ubiquitination and degradation and FLNB which prevents its ability to interact with the upstream activators of the JNK cascade therby inhibiting IFNA-induced JNK signaling. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A, HIV-1 and Ebola virus. Restricts HIV-1 and ebola virus via disruption of viral budding. Inhibits the ubiquitination of HIV-1 Gag and host TSG101 and disrupts their interaction, thereby preventing assembly and release of virions from infected cells. Inhibits Ebola virus budding mediated by the VP40 protein by disrupting ubiquitin ligase activity of NEDD4 and its ability to ubiquitinate VP40. ISGylates influenza A virus NS1 protein which causes a loss of function of the protein and the inhibition of virus replication. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity.

亚细胞位置:

Cytoplasm Secreted Exists in three distinct states: free within the cell, released into the extracellular space, or conjugated to target proteins.

数据库链接

UniGene:

Hs.458485

SMR:

P05161

STRING:

9606.ENSP00000368699

KEGG:

hsa:9636

MIM: