首页 > 重组蛋白 > Human CD63 Recombinant Protein

CD63 (基因名), CD63 antigen (蛋白名), cd63_human.

产品名称:

Human CD63/ CD63 antigen Recombinant Protein
溶酶体相关膜蛋白3

货号:

R9307h

商标:

EIAab^®

监管等级:

RUO

别名:

Granulophysin, Lysosomal-associated membrane protein 3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, LAMP-3, Tspan-30, CD63, MLA1, TSPAN30

序列号:

P08962

来源:

E.coli

种属:

Human

标签:

His

序列:

103-203aa

预估分子量:

11.11 kDa (monomer)

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

规格 & 价格:

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Human CD63 Protein

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产品说明书

说明书:

MSDS:

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数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

The PCR product of human CD63 gene was determined by 1% Agarose stained with EB.

Recombinant human CD63 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 12q13.2 by HGNC 12q13.2 by Entrez Gene 12q13.2 by Ensembl

CD63 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with TIMP1 and ITGB1 and recruits TIMP1 to ITGB1 (PubMed:16917503, PubMed:24635319). Interacts with CD9. Identified in a complex with CD9 and ITGB3 (PubMed:19640571). Interacts with PMEL (PubMed:21962903). Interacts with KDR/VEGFR2; identified in a complex with ITGB1 and KDR/VEGFR2 and is required to recruit KDR to ITGB1 complexes (PubMed:23632027). Interacts with SYT7.

功能:

Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.

亚细胞位置:

Cell membrane Multi-pass membrane protein Lysosome membrane Multi-pass membrane protein Late endosome membrane Multi-pass membrane protein Endosome Multivesicular body Melanosome Secreted Exosome Cell surface Also found in Weibel-Palade bodies of endothelial cells (PubMed:10793155). Located in platelet dense granules (PubMed:7682577). Detected in a subset of pre-melanosomes. Detected on intralumenal vesicles (ILVs) within multivesicular bodies (PubMed:21962903).

数据库链接

UniGene:

Hs.445570

STRING:

9606.ENSP00000257857

KEGG:

hsa:967

MIM:

155740

Pfam:

PF00335

Uniprot:

P08962

相关产品

Mouse Cd63 ELISA Kit

Rat Cd63 ELISA Kit

Bovine CD63 ELISA Kit

Mouse Cd63 CLIA Kit

Rat Cd63 CLIA Kit

序列长度:

238

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

238:

KNVLVVAAAA | LGIAFVEVLG | IVFACCLVKS | IRSGYEVM

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"The protein CD63 is in platelet dense granules, is deficient in a patient with Hermansky-Pudlak syndrome, and appears identical to granulophysin."

(PMID: 7682577)

[2].

"An ocular melanoma-associated antigen. Molecular characterization."

(PMID: 1339263)

[3].

"TIMP-1 modulates chemotaxis of human neural stem cells through CD63 and integrin signalling."

(PMID: 24635319)

[4].

"Tetraspanin CD63 promotes vascular endothelial growth factor receptor 2-β1 integrin complex formation, thereby regulating activation and downstream signaling in endothelial cells in vitro and in vivo."

(PMID: 23632027)

[5].

"Syndecan-syntenin-ALIX regulates the biogenesis of exosomes."

(PMID: 22660413)

[6].

"The role of ceroid lipofuscinosis neuronal protein 5 (CLN5) in endosomal sorting."

(PMID: 22431521)

[7].

"CD63 is an essential cofactor to leukocyte recruitment by endothelial P-selectin."

(PMID: 21803846)

[8].

"The tetraspanin CD63 regulates ESCRT-independent and -dependent endosomal sorting during melanogenesis."

(PMID: 21962903)

[9].

"Palmitoylation supports the association of tetraspanin CD63 with CD9 and integrin alphaIIbbeta3 in activated platelets."

(PMID: 19640571)

[10].

"Tetraspanins regulate cell-to-cell transmission of HIV-1."

(PMID: 19602278)

[1].

人溶酶体相关膜蛋白3(CD63)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人溶酶体相关膜蛋白3(CD63)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人溶酶体相关膜蛋白3(CD63)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人溶酶体相关膜蛋白3(CD63)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人溶酶体相关膜蛋白3(CD63)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人溶酶体相关膜蛋白3(CD63)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人溶酶体相关膜蛋白3(CD63)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人溶酶体相关膜蛋白3(CD63)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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