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Pdk1 (基因名), [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (蛋白名), pdk1_rat.
产品名称:

Rat Pdk1/ [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial ELISA Kit
丙酮酸脱氢酶[硫辛酰胺]激酶同功酶1

货号:

E10511r

商标:
EIAab®
监管等级:
别名:

PDK p48, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1, Pdh

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.156-10ng/mL
灵敏度:
0.081ng/mL
特异性:
Natural and recombinant rat [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Cancer
Rat Pdk1 ELISA Kit
规格 & 价格: cart
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Rat Pdk1 ELISA Kit
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产品说明书
说明书: 下载说明书
MSDS: MSDS
在线询价


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤4.3%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤8.1%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

95

89-101

Plasma

97

91-103

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

92-104%

105-115%

113-123%

89-97%

EDTA plasma(n=5)

89-99%

86-96%

107-117%

93-102%

heparin plasma(n=5)

106-115%

 

102-111%

104-114%

96-106%

 

通用注释


亚单元:
Homodimer, and heterodimer with PDK2. Interacts with the pyruvate dehydrogenase complex subunit DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3).


功能:
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.


亚细胞位置:
Mitochondrion matrix


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