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Ilk (基因名), Integrin-linked protein kinase (蛋白名), ilk_rat.
产品名称:

Rat Ilk/ Integrin-linked protein kinase ELISA Kit
整合素连接蛋白激酶

货号:

E1587r

商标:
EIAab®
监管等级:
检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.156-10ng/ml
灵敏度:
0.078ng/mL
特异性:
Natural and recombinant rat Integrin-linked protein kinase
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Cancer
Rat Ilk ELISA Kit
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产品说明书
说明书: 下载说明书
MSDS: MSDS
在线询价


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤4.5%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤8.2%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

101

95-107

Plasma

103

97-109

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

116-126%

90-99%

110-120%

97-107%

EDTA plasma(n=5)

100-110%

84-95%

102-112%

103-116%

heparin plasma(n=5)

97-107%

 

90-100%

110-120%

84-94%

 

通用注释


亚单元:
Interacts with FERMT2 (By similarity). Interacts with the cytoplasmic domain of ITGB1. Could also interact with integrin ITGB2, ITGB3 and/or ITGB5. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with PARVA and PARVB; these compete for the same binding site. Interacts probably also with TGFB1I1. Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1. Interacts with LIMD2; leading to activate the protein kinase activity.


功能:
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.


亚细胞位置:
Cell junction Focal adhesion Cell membrane Peripheral membrane protein Cytoplasmic side Cell projection Lamellipodium Cytoplasm Myofibril Sarcomere


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