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CDH5 (基因名), Cadherin-5 (蛋白名), cadh5_human.
产品名称:

Human CDH5/ Cadherin-5 ELISA Kit
钙粘蛋白5

货号:

E1366h

商标:
EIAab®
监管等级:
别名:

7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CD144

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.156-10ng/mL
灵敏度:
0.1ng/mL
特异性:
Natural and recombinant human Cadherin-5
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Development Biology
Human CDH5 ELISA Kit
规格 & 价格: cart
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Human CDH5 ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS
在线询价


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤5.3%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤9.4%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

99

93-105

Plasma

101

95-107

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

95-104%

91-101%

110-119%

95-105%

EDTA plasma(n=5)

96-106%

93-104%

101-111%

103-112%

heparin plasma(n=5)

112-122%

 

104-104%

101-112%

89-99%

 

通用注释


亚单元:
Interacts (via cadherin 5 domain) with PTPRB (By similarity). Interacts with TRPC4. Interacts with KRIT1. Interacts with PARD3.


功能:
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.


亚细胞位置:
Cell junction Cell membrane Single-pass type I membrane protein Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions.


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