首页 > 重组蛋白 > Human A1CF Recombinant Protein

A1CF (基因名), APOBEC1 complementation factor (蛋白名), A1CF_HUMAN.

产品名称:

Human A1CF/ APOBEC1 complementation factor Recombinant Protein

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

APOBEC1-stimulating protein, ACF, ASP

序列号:

Q9NQ94

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

关注

Human A1CF Protein

邮箱 *

消息 *

Please 登录.

产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
反馈墙 (0)

基因位点

Cytogenetic band: 10q11.23 by HGNC 10q11.23 by Entrez Gene 10q11.23 by Ensembl

A1CF Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Part of the apolipoprotein B mRNA editing complex with APOBEC1. Interacts with TNPO2; TNPO2 may be responsible for transport of A1CF into the nucleus. Interacts with SYNCRIP. Interacts with CELF2/CUGBP2.

功能:

Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay.

亚细胞位置:

Nucleus Endoplasmic reticulum Cytoplasm Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum (By similarity). Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1.

数据库链接

UniGene:

Hs.282795

SMR:

Q9NQ94

STRING:

9606.ENSP00000282641

KEGG:

hsa:29974

Pfam:

PF00076

Uniprot:

Q9NQ94

您可能感兴趣

Human A1BG ELISA Kit

Mouse A1bg ELISA Kit

Rat A1bg ELISA Kit

Bovine A1BG ELISA Kit

Pig A1BG ELISA Kit

序列长度:

594

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

594:

ATAPVSAAQL | KQAVTLGQDL | AAYTTYEVYP | TFAVTARGDG | YGTF

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"The apolipoprotein B mRNA editing complex performs a multifunctional cycle and suppresses nonsense-mediated decay."

(PMID: 12881431)

[2].

"A novel nuclear localization signal in the auxiliary domain of apobec-1 complementation factor regulates nucleocytoplasmic import and shuttling."

(PMID: 12896982)

[3].

"Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing."

(PMID: 11815617)

[4].

"Identification of domains in apobec-1 complementation factor required for RNA binding and apolipoprotein-B mRNA editing."

(PMID: 11871661)

[5].

"Isolation, characterization and developmental regulation of the human apobec-1 complementation factor (ACF) gene."

(PMID: 11718896)

[6].

"Molecular cloning of apobec-1 complementation factor, a novel RNA-binding protein involved in the editing of apolipoprotein B mRNA."

(PMID: 10669759)

[7].

"Targeted deletion of the murine apobec-1 complementation factor (acf) gene results in embryonic lethality."

(PMID: 16055734)

[8].

"Complete sequencing and characterization of 21,243 full-length human cDNAs."

(PMID: 14702039)

[9].

"The DNA sequence and comparative analysis of human chromosome 10."

(PMID: 15164054)

[10].

"Identification of GRY-RBP as an apolipoprotein B RNA-binding protein that interacts with both apobec-1 and apobec-1 complementation factor to modulate C to U editing."

(PMID: 11134005)

[1].

人A1CF重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人A1CF重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人A1CF重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人A1CF重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人A1CF重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人A1CF重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人A1CF重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人A1CF重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

反馈墙

评论数 : 0

所有用户

默认排序