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Nfkb1 (基因名), Nuclear factor NF-kappa-B p105 subunit (蛋白名), nfkb1_rat.

产品名称:

Rat Nfkb1/ Nuclear factor NF-kappa-B p105 subunit ELISA Kit
核因子κ-Bp105亚基

货号:

E1824r

商标:

EIAab^®

监管等级:

RUO

别名:

DNA-binding factor KBF1, EBP-1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.156-10ng/mL

灵敏度:

0.092ng/mL

特异性:

Natural and recombinant rat Nuclear factor NF-kappa-B p105 subunit

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Immunology

规格 & 价格:

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产品说明书

数据表:

说明书:

MSDS:

在线询价

数据
文献 ( 39 )
序列 / 3D
常见问题解答
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精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤4.2%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤7.8%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	99	93-105
Plasma	101	95-107

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	84-96%	115-125%	94-104%	114-124%
EDTA plasma(n=5)	101-110%	108-118%	105-115%	93-102%
heparin plasma(n=5)	83-95%	106-117%	94-104%	107-117%

通用注释

亚单元:

Component of the NF-kappa-B p65-p50 complex. Component of the NF-kappa-B p65-p50 complex. Homodimer; component of the NF-kappa-B p50-p50 complex. Component of the NF-kappa-B p105-p50 complex. Component of the NF-kappa-B p50-c-Rel complex. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Also interacts with MAP3K8. NF-kappa-B p50 subunit interacts with NCOA3 coactivator, which may coactivate NF-kappa-B dependent expression via its histone acetyltransferase activity. Interacts with DSIPI; this interaction prevents nuclear translocation and DNA-binding. Interacts with SPAG9 and UNC5CL. NFKB1/p105 interacts with CFLAR; the interaction inhibits p105 processing into p50. NFKB1/p105 forms a ternary complex with MAP3K8 and TNIP2. Interacts with GSK3B; the interaction prevents processing of p105 to p50. NFKB1/p50 interacts with NFKBIE. NFKB1/p50 interacts with NFKBIZ. Nuclear factor NF-kappa-B p50 subunit interacts with NFKBID (By similarity). Directly interacts with MEN1 (By similarity). Interacts with HIF1AN.

功能:

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. Plays a role in the regulation of apoptosis. Isoform 5, isoform 6 and isoform 7 act as inhibitors of transactivation of p50 NF-kappa-B subunit, probably by sequestering it in the cytoplasm. Isoform 3 (p98) (but not p84 or p105) acts as a transactivator of NF-kappa-B-regulated gene expression. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.

亚细胞位置:

Nucleus Cytoplasm Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).

数据库链接

UniGene:

Rn.2411

SMR:

Q63369

STRING:

10116.ENSRNOP00000028944

Pfam:

PF12796

Uniprot:

Q63369

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Human NFKB1 CLIA Kit

序列长度:

522

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

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500:

522:

PLLSLNKMPH | NYGQDGPIEG | KI

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Vanillin as a new modulator candidate for renal injury induced by cisplatin in experimental rats"

M M.Elseweidy, S E.Elswefy, M Shawky, et al.

From: Cytokine

[2].

"Effect of cardamonin on hepatic ischemia reperfusion induced in rats: Role of nitric oxide"

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From: European Journal of Pharmacology

[3].

"Nephrotoxicity Induced by Cisplatin Intake in Experimental Rats and Therapeutic Approach of Using Mesenchymal Stem Cells and Spironolactone"

M M.Elseweidy, S E.Elswefy, M Shawky, et al.

From: Applied Biochemistry and Biotechnology

[4].

"Potential neuroprotective effect of androst-5-ene-3β, 17β-diol (ADIOL) on the striatum, and substantia nigra in Parkinson's disease rat model"

R M.Salama, M G.Tadros, Nevine Bahaa, et al.

From: Cellular Physiology

[5].

"Cilostazol attenuates indices of liver damage induced by thioacetamide in albino rats through regulating inflammatory cytokines and apoptotic biomarkers"

S A. Awdan, M M. Amin, A Hassan.

From: European Journal of Pharmacology

[6].

"Plumbagin ameliorates hepatic ischemia-reperfusion injury in rats: Role of high mobility group box 1 in inflammation, oxidative stress and apoptosis"

A M. Zaki, D M. El-Tanbouly, H F. Zaki, et al.

From: Biomedicine & Pharmacotherapy

[7].

"Montelukast attenuates rotenone-induced microglial activation/p38 MAPK expression in rats: Possible role of its antioxidant, anti-inflammatory and antiapoptotic effects"

R M. Mansour, M A.E. Ahmed, A E. EI-Sahar, et al.

From: Toxicology and Applied Pharmacology

[8].

"Montelukast abrogates prednisolone-induced hepatic injury in rats: Modulation of mitochondrial dysfunction, oxidative/nitrosative stress, and apoptosis"

I I Hegab, H E El-Horany, M M. Elbatsh, et al.

From: Journal of Biochemical and Molecular Toxicology

[9].

"Protective role of chrysin on thioacetamide-induced hepatic encephalopathy in rats"

S A. El-Marasy, S A. El Awdan, R M. Abd-Elsalam.

From: Chemico-Biological Interactions

[10].

"Pharmacological Manipulation of Trk, p75NTR, and NGF Balance Restores Memory Deficit in Global Ischemia/Reperfusion Model in Rats"

A M Choucry, M Y Al-Shorbagy, A S Attia, et al.

From: Journal of Molecular Neuroscience

[11].

"Olmesartan attenuates type 2 diabetes-associated liver injury: Cross-talk of AGE/RAGE/JNK, STAT3/SCOS3 and RAS signaling pathways"

D O Saleh, S S. Mahmoud, S M. Nofal, et al.

From: European Journal of Pharmacology

[12].

"Phytochemical profile and protective effect of Ocimum basilicum aqueous extract in doxorubicin/irradiation‐induced testicular injury"

R Y. M. Ibrahim, S M. Mansour, W M. Elkady.

From: Journal of Pharmacy and Pharmacology

[13].

"Lycopene induces insulin signaling and alleviates fibrosis in experimental model of non-alcoholic fatty liver disease in rats"

N M. Saeed, A M. Mansour, S Allam.

From: Pharma Nutrition

[14].

"The modulatory effects of luteolin on cyclic AMP/Ciliary neurotrophic factor signaling pathway in experimentally induced autoimmune encephalomyelitis"

O S El‐Deeb, H B Ghanem, R O El‐Esawy, et al.

From: IUBMB LIFE

[15].

"Molecular mechanisms of neuroprotective effect of adjuvant therapy with phenytoin in pentylenetetrazole-induced seizures: Impact on Sirt1/NRF2 signaling pathways"

M M. Nagib, M G. Tadros, R M. Rahmo, et al.

From: NeuroToxicology

[16].

"Beta-caryophyllene protects against diet-induced dyslipidemia and vascular inflammation in rats: Involvement of CB2 and PPAR-γ receptors"

D A. Youssef, H M. El-Fayoumi, M F. Mahmoud.

From: Chemico-Biological Interactions

[17].

"Role of pERK1/2-NFκB signaling in the neuroprotective effect of thalidomide against cerebral ischemia reperfusion injury in rats"

R A. Mohammed, M F. El-Yamany, A A. Abdel-Rahman, et al.

From: European Journal of Pharmacology

[18].

"Pre-treatment with cardamonin protects against cisplatin-induced nephrotoxicity in rats: Impact on NOX-1, inflammation and apoptosis"

Reem N. EI-Naga

From: Toxicology and applied pharmacology

[19].

"Inhibitory effect of ginsenoside-Rd on carrageenan-induced inflammation in rats"

L Wang, Y Zhang, Z Wang, et al.

From: Canadian journal of physiology and pharmacology

[20].

"Pinocembrin attenuates hippocampal inflammation, oxidative perturbations and apoptosis in a rat model of global cerebral ischemia reperfusion"

M Saad, R Abdel Salam, S Kenawy, et al.

From: Pharmacological Reports

[21].

"Vitamin D ameliorates hepatic ischemic/reperfusion injury in rats"

AA Seif , DM Abdelwahed

From: J Physiol Biochem

[22].

"Montelukast, a Cysteinyl Leukotriene Receptor-1 Antagonist Protects Against Hippocampal Injury Induced by Transient Global Cerebral Ischemia..."

M. A. Saad; R. M. Abdelsalam; S. A. Kenawy, et al.

From: Neurochemical Research

[23].

"Sitagliptin attenuates transient cerebral ischemia/reperfusion injury in diabetic rats: Implication of the oxidative–inflammatory–apoptotic pathway"

AE El-Sahar, MM Safar, HF Zaki, et al.

From: Life Sciences

[24].

"Ischemic preconditioning and postconditioning alleviates hippocampal tissue damage through abrogation of apoptosis modulated by oxidative stress ..."

M A. Saad, R M. Abdel Salam, S A. Kenawy, et al.

From: Chemico-Biological Interactions

[25].

"Neuroprotective effects of vildagliptin in rat rotenone Parkinson’s disease model: role of RAGE-NFκB and Nrf2-antioxidant signaling ..."

RM Abdelsalam, MM Safar

From: Journal of Neurochemistry

[26].

"Molecular mechanisms of the protective role of wheat germ oil against cyclosporin A-induced hepatotoxicity in rats"

ES Akool

From: Pharmaceutical Biology

[27].

"Neuroprotective effects of pioglitazone against transient cerebral ischemic reperfusion injury in diabetic rats: Modulation of antioxidant ..."

AE El-Sahar, MM Safar, HF Zaki, et al.

From: Pharmacological Reports

[28].

"Molecular Mechanisms of the Modulatory Effect of Vitamin E on Tacrolimus (FK506)-Induced Renal Injury in Rats"

H Afify, AAH Abdel-Aziz, MN El-Din, et al.

From: British Journal of Pharmaceutical Research

[29].

"10-DHGD ameliorates cisplatin-induced nephrotoxicity in rats"

Elseweidy M M, Zaghloul M S, Younis N N.

From: Biomedicine & Pharmacotherapy

[30].

"Neuroprotective Effect of Nanodiamond in Alzheimer’s Disease Rat Model: a Pivotal Role for Modulating NF-κB and STAT3 Signaling"

Alawdi S H, El-Denshary E S, Safar M M, et al.

From: Molecular neurobiology

[31].

"Quercetin protects against thioacetamide induced hepatotoxicity in rats through decreased oxidative stress biomarkers, the inflammatory cytokines ..."

Afifi N A, Ramadan A, El-Eraky W, et al.

From: DER PHARMA CHEMICA

[32].

"Role of adenosine A2A receptor in cerebral ischemia reperfusion injury: Signaling to phosphorylated extracellular signal-regulated protein kinase ..."

Al-Daghri N M, Rahman S, Sabico S, et al.

From: Journal of Diabetes and its Complications

[33].

"A new combination of sitagliptin and furosemide protects against remote myocardial injury induced by renal ischemia/reperfusion in rats"

Youssef M I, Mahmoud A A A, Abdelghany R H.

From: Biochemical pharmacology

[34].

"Geraniol ameliorates TNBS-induced colitis: Involvement of Wnt/β-catenin, p38MAPK, NFκB, and PPARγ signaling pathways"

Soubh A A, Abdallah D M, El-Abhar H S.

From: Life sciences

[35].

"Paeoniflorin ameliorates acute myocardial infarction of rats by inhibiting inflammation and inducible nitric oxide synthase signaling pathways"

Chen C, Du P, Wang J.

From: Molecular medicine reports

[36].

"Aspirin or omega-3 polyunsaturated fatty acids protects against cortico-hippocampal neurodegeneration and downregulates lipoxin A..."

Abd-Elghafour B A, El-Sayed N M, Ahmed A A M, et al

From: Canadian Journal of Physiology and Pharmacology

[37].

"Estimation of Ellagic acid and/or Repaglinide Effects on Insulin Signaling, Oxidative Stress and Inflammatory Mediators of Liver, Pancreas, Adipose..."

Amin M M, Arbid M S.

From: Applied Physiology, Nutrition, and Metabolism

[38].

"Protective effect of cardamonin against acetic acid-induced ulcerative colitis in rats"

AA Ali, ENAA Haleem, SA Khaleel, et al.

From: Pharmacological Reports

[39].

"Beta-caryophyllene alleviates diet-induced neurobehavioral changes in rats: The role of CB2 and PPAR-γ receptors"

D A. Youssef, H M. El-Fayoumi, M F. Mahmoud.

From: Biomedicine & Pharmacotherapy

[1].

大鼠核因子κ-Bp105亚基(Nfkb1)ELISA试剂盒可以做多少个样本？

大鼠核因子κ-Bp105亚基(Nfkb1)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

大鼠核因子κ-Bp105亚基(Nfkb1)ELISA试剂盒使用视频？

大鼠核因子κ-Bp105亚基(Nfkb1)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

大鼠核因子κ-Bp105亚基(Nfkb1)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的大鼠核因子κ-Bp105亚基(Nfkb1)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

大鼠核因子κ-Bp105亚基(Nfkb1)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

大鼠核因子κ-Bp105亚基(Nfkb1)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析大鼠核因子κ-Bp105亚基(Nfkb1)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

大鼠核因子κ-Bp105亚基(Nfkb1)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集大鼠核因子κ-Bp105亚基(Nfkb1)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

大鼠核因子κ-Bp105亚基(Nfkb1)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

大鼠核因子κ-Bp105亚基(Nfkb1)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

大鼠核因子κ-Bp105亚基(Nfkb1)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Rat Nfkb1/ Nuclear factor NF-kappa-B p105 subunit ELISA Kit 核因子κ-Bp105亚基

E1824r

DNA-binding factor KBF1, EBP-1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Rat Nfkb1/ Nuclear factor NF-kappa-B p105 subunit ELISA Kit
核因子κ-Bp105亚基