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EZH2 (基因名), Histone-lysine N-methyltransferase EZH2 (蛋白名), ezh2_human.

产品名称:

Human EZH2/ Histone-lysine N-methyltransferase EZH2 ELISA Kit
Histone-lysine N-methyltransferase EZH2

货号:

E7688h

商标:

EIAab^®

监管等级:

RUO

别名:

ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, KMT6

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.78 -50 ng/mL

灵敏度:

0.24 ng/mL

特异性:

Natural and recombinant human Histone-lysine N-methyltransferase EZH2

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Epigenetics

规格 & 价格:

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产品说明书

数据表:

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-101%	109-118%	113-122%	86-98%
EDTA plasma(n=5)	90-100%	109-119%	83-93%	80-90%
heparin plasma(n=5)	92-102%	98-109%	96-104%	101-111%

通用注释

亚单元:

Binds ATRX via the SET domain (Probable). Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC1 and HDAC2. Interacts with PRAME. Interacts with CDYL. Interacts with CLOCK, ARNTL/BMAL1 and CRY1 (By similarity). Interacts with DNMT3L; the interaction is direct.

功能:

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.

亚细胞位置:

Nucleus

数据库链接

UniGene:

Hs.444082

SMR:

Q15910

STRING:

9606.ENSP00000320147

KEGG:

hsa:2146

MIM:

277590

Pfam:

PF11616

Uniprot:

Q15910

相关产品

Mouse Ezh2 ELISA Kit

Mouse Ezh2 CLIA Kit

Mouse Ezh2 Protein

Mouse Ezh2 Polyclonal Antibody

Mouse Ezh2 Monoclonal Antibody

序列长度:

746

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

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250:

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650:

700:

746:

MVNGDHRIGI | FAKRAIQTGE | ELFFDYRYSQ | ADALKYVGIE | REMEIP

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

人Histone-lysine N-methyltransferase EZH2(EZH2)ELISA试剂盒可以做多少个样本？

人Histone-lysine N-methyltransferase EZH2(EZH2)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人Histone-lysine N-methyltransferase EZH2(EZH2)ELISA试剂盒使用视频？

人Histone-lysine N-methyltransferase EZH2(EZH2)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人Histone-lysine N-methyltransferase EZH2(EZH2)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人Histone-lysine N-methyltransferase EZH2(EZH2)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人Histone-lysine N-methyltransferase EZH2(EZH2)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人Histone-lysine N-methyltransferase EZH2(EZH2)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人Histone-lysine N-methyltransferase EZH2(EZH2)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人Histone-lysine N-methyltransferase EZH2(EZH2)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人Histone-lysine N-methyltransferase EZH2(EZH2)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人Histone-lysine N-methyltransferase EZH2(EZH2)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人Histone-lysine N-methyltransferase EZH2(EZH2)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人Histone-lysine N-methyltransferase EZH2(EZH2)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Human EZH2/ Histone-lysine N-methyltransferase EZH2 ELISA Kit Histone-lysine N-methyltransferase EZH2

E7688h

ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, KMT6

Human EZH2/ Histone-lysine N-methyltransferase EZH2 ELISA Kit
Histone-lysine N-methyltransferase EZH2