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AKT2 (基因名), RAC-beta serine/threonine-protein kinase (蛋白名), akt2_human.
产品名称:

Human AKT2/ RAC-beta serine/threonine-protein kinase ELISA Kit
RACβ丝氨酸/苏氨酸蛋白激

货号:

E9360h

商标:
EIAab®
监管等级:
别名:

Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, PKB beta

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
78-5000pg/mL
灵敏度:
39.8pg/mL
特异性:
Natural and recombinant human RAC-beta serine/threonine-protein kinase
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Cancer
Human AKT2 ELISA Kit
规格 & 价格: cart
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Human AKT2 ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS
在线询价


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤6.7%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤8.0%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

93

87-99

Plasma

95

89-101

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

95-105%

88-99%

89-99%

106-116%

EDTA plasma(n=5)

107-117%

96-106%

107-116%

97-106%

heparin plasma(n=5)

95-104%

 

104-114%

98-108%

100-109%

 

通用注释


亚单元:
Interacts with BTBD10 (By similarity). Interacts with KCTD20 (By similarity). Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CLK2, PBH2 and TRAF6. Interacts (when phosphorylated) with CLIP3, the interaction promotes cell membrane localization (PubMed:19139280). Interacts with WDFY2 (via WD repeats 1-3) (PubMed:16792529).


功能:
One of the few specific substrates of AKT2 identified recently is PITX2. Phosphorylation of PITX2 impairs its association with the CCND1 mRNA-stabilizing complex thus shortening the half-life of CCND1. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. Phosphorylates C2CD5 on 'Ser-197' during insulin-stimulated adipocytes. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis. Phosphorylates CLK2 on 'Thr-343'.


亚细胞位置:
Cytoplasm Nucleus Cell membrane Peripheral membrane protein Early endosome Localizes within both nucleus and cytoplasm of proliferative primary myoblasts and mostly within the nucleus of differentiated primary myoblasts. By virtue of the N-terminal PH domain, is recruited to sites of the plasma membrane containing increased PI(3,4,5)P3 or PI(3,4)P2, cell membrane targeting is also facilitared by interaction with CLIP3. Colocalizes with WDFY2 in early endosomes (By similarity).


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