Akt1 (基因名), RAC-alpha serine/threonine-protein kinase (蛋白名), akt1_rat.
产品名称:
Rat Akt1/ RAC-alpha serine/threonine-protein kinase ELISA Kit
α-丝氨酸/苏氨酸蛋白激酶
货号:
E0382r
商标:
EIAab®
监管等级:
别名:
Protein kinase B, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB alpha
检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.156-10ng/ml
灵敏度:
0.078ng/mL
特异性:
Natural and recombinant rat RAC-alpha serine/threonine-protein kinase
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
登录.
实验步骤:
研究领域:
Cancer
精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤4.6%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤7.8%
批内 CV: ≤4.6%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤7.8%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
95 |
89-101 |
Plasma |
97 |
91-103 |
线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
99-109% |
87-99% |
108-117% |
82-92% |
EDTA plasma(n=5) |
103-113% |
116-126% |
98-108% |
91-100% |
heparin plasma(n=5) |
107-117%
|
86-95% |
91-101% |
90-100% |
通用注释
亚单元:
Interacts (via the C-terminus) with CCDC88A (via its C-terminus). Interacts with GRB10; the interaction leads to GRB10 phosphorylation thus promoting YWHAE-binding. Interacts with AGAP2 (isoform 2/PIKE-A); the interaction occurs in the presence of guanine nucleotides. Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CDKN1B; the interaction phosphorylates CDKN1B promoting 14-3-3 binding and cell-cycle progression. Interacts with MAP3K5 and TRAF6. Interacts with BAD, PPP2R5B, STK3 and STK4. Interacts (via PH domain) with SIRT1. Interacts with SRPK2 in a phosphorylation-dependent manner. Interacts with TNK2 and CLK2. Interacts with RAF1. Interacts (via the C-terminus) with THEM4 (via its C-terminus). Interacts with TRIM13; the interaction ubiquitinates AKT1 leading to its proteasomal degradation. Interacts with and phosphorylated by PDPK1 (By similarity). Interacts with BTBD10 (By similarity). Interacts with KCTD20 (By similarity). Interacts with PA2G4. Interacts with KIF14; the interaction is detected in the plasma membrane upon INS stimulation and promotes AKT1 phosphorylation (By similarity). Interacts with FAM83B; activates the PI3K/AKT signaling cascade (By similarity). Interacts with WDFY2 (via WD repeats 1-3) (By similarity). Forms a complex with WDFY2 and FOXO1 (By similarity). Interacts with FAM168A.
功能:
AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.
亚细胞位置:
Cytoplasm
Nucleus
Cell membrane
Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A (By similarity). Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles (By similarity).
该产品尚未在任何出版物中被引用。
[1].
大鼠α-丝氨酸/苏氨酸蛋白激酶(Akt1)ELISA试剂盒可以做多少个样本?
大鼠α-丝氨酸/苏氨酸蛋白激酶(Akt1)ELISA试剂盒分为2种规格,96孔和48孔。96孔的试剂盒,标曲和样本都做复孔的话,可以检测40个样本。96孔的试剂盒,标曲和样本都不做复孔的话,可以检测88个样本。
[2].
大鼠α-丝氨酸/苏氨酸蛋白激酶(Akt1)ELISA试剂盒使用视频?
大鼠α-丝氨酸/苏氨酸蛋白激酶(Akt1)ELISA试剂盒实验操作视频在以下网址中,对每一步的实验步骤都做了演示,方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html
https://www.eiaab.com.cn/lesson-tech/805.html
[3].
大鼠α-丝氨酸/苏氨酸蛋白激酶(Akt1)ELISA试剂盒是放在-20℃冰箱保存吗?
EIAab的大鼠α-丝氨酸/苏氨酸蛋白激酶(Akt1)ELISA试剂盒,洗涤液、底物、终止液保存于4℃,其余试剂-20℃冰箱保存。
[4].
大鼠α-丝氨酸/苏氨酸蛋白激酶(Akt1)ELISA试剂盒原理?
双抗体夹心法:用纯化的抗体包被微孔板,制成固相抗体,往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
竞争法:用纯化的抗体包被微孔板,制成固相抗体,往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物,受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
竞争法:用纯化的抗体包被微孔板,制成固相抗体,往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物,受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
[5].
大鼠α-丝氨酸/苏氨酸蛋白激酶(Akt1)ELISA试剂盒中需要使用的样品量是多少?
夹心法100μL/孔,竞争法50μL/孔。如样本浓度过高时,应对样本进行稀释,以使稀释后的样本符合试剂盒的检测范围,计算时再乘以相应的稀释倍数。
[6].
如何分析大鼠α-丝氨酸/苏氨酸蛋白激酶(Akt1)ELISA试剂盒数据?
建议标准曲线,并计算样本浓度。对于elisa的曲线拟合,一般建议采用4参数曲线拟合,4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合,例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度,再乘以稀释倍数;或用标准物的浓度与OD值计算出标曲的回归方程式,将样本的OD值代入方程式,计算出样本浓度,再乘以稀释倍数,即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/
https://www.eiaab.com.cn/news/502/
[7].
大鼠α-丝氨酸/苏氨酸蛋白激酶(Akt1)ELISA试剂盒中是否包含人和动物的副产物,是否包含感染的或者传染性原料如HIV等?
除了抗体和稀释液中的BSA,不含其它人和动物的副产物,也不含感染材料。
[8].
收集大鼠α-丝氨酸/苏氨酸蛋白激酶(Akt1)ELISA试剂盒血浆样本,用什么作为抗凝剂?
一般建议用EDTA和肝素作为抗凝剂。
[9].
大鼠α-丝氨酸/苏氨酸蛋白激酶(Akt1)ELISA试剂盒酶标板可以拆成几部分?拆的时候是否需要避光,无菌?
大鼠α-丝氨酸/苏氨酸蛋白激酶(Akt1)ELISA试剂盒酶标板是8×12孔条,可拆卸,板子可以拆成12条,注意避免孔污染,不需要避光和无菌。暂时不用的板子,放回原来装的袋子里,密封保存。
[10].
大鼠α-丝氨酸/苏氨酸蛋白激酶(Akt1)ELISA试剂盒样本如何保存?
尽量检测新鲜样本。若无新鲜样本,则4℃保存1周,-20℃保存1个月,-80℃保存2个月。
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