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首页  >  酶联免疫试剂盒  >  Human MMP13 ELISA Kit
MMP13 (基因名), Collagenase 3 (蛋白名), MMP13_HUMAN.
产品名称:

Human MMP13/ Collagenase 3 ELISA Kit
胶原蛋白酶3

货号:

E0099h

商标:
EIAab®
监管等级:
别名:

Matrix metalloproteinase-13, MMP-13

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.156-10ng/mL
灵敏度:
0.039ng/mL
特异性:
Natural and recombinant human Collagenase 3
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Cancer
Human MMP13 ELISA Kit
规格 & 价格: cart
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Human MMP13 ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤4.8%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤9.6%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

100

94-106

Plasma

102

96-108

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

100-110%

99-109%

83-96%

91-102%

EDTA plasma(n=5)

108-118%

94-104%

105-114%

106-117%

heparin plasma(n=5)

84-93%

 

105-113%

104-114%

87-97%

 

通用注释


亚单元:
Monomer. Interacts with TIMP1, TIMP2 and TIMP3. Binds (via the C-terminal region) to collagen.


功能:
Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.


亚细胞位置:
Secreted Extracellular space Extracellular matrix Secreted


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