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首页  >  酶联免疫试剂盒  >  Mouse Map1lc3a ELISA Kit
Map1lc3a (基因名), Microtubule-associated proteins 1A/1B light chain 3A (蛋白名), MLP3A_MOUSE.
产品名称:

Mouse Map1lc3a/ Microtubule-associated proteins 1A/1B light chain 3A ELISA Kit
HumanMicrotubule-associatedproteins1A/1Blightchain3A

货号:

E2879m

商标:
EIAab®
监管等级:
别名:

Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.78-50ng/mL
灵敏度:
0.41ng/mL
特异性:
Natural and recombinant mouse Microtubule-associated proteins 1A/1B light chain 3A
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Cancer
Mouse Map1lc3a ELISA Kit
规格 & 价格: cart
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Mouse Map1lc3a ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤6.5%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤7.9%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

88

82-94

Plasma

90

84-96

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

106-116%

97-108%

102-114%

100-100%

EDTA plasma(n=5)

88-100%

107-115%

108-119%

89-89%

heparin plasma(n=5)

111-120%

 

89-99%

90-98%

81-90%

 

通用注释


亚单元:
3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins (By similarity). Interacts with TP53INP1 and TP53INP2. Directly interacts with SQSTM1; this interaction leads to MAP1LC3A recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy. Interacts with ATG13. Interacts with ULK1. Interacts with TBC1D5. Found in a complex with UBQLN1 and UBQLN2. Interacts with UBQLN4 (via STI1 1 and 2 domains). Interacts with UBQLN1 in the presence of UBQLN4. Interacts with TRIM5. Interacts with MEFV. Interacts with FAM134A, FAM134B and FAM134C.


功能:
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.


亚细胞位置:
Cytoplasm Cytoskeleton Endomembrane system Lipid-anchor Cytoplasmic vesicle Autophagosome membrane Lipid-anchor Cytoplasmic vesicle Autophagosome LC3-II binds to the autophagic membranes.


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