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首页  >  酶联免疫试剂盒  >  Human MED31 ELISA Kit
MED31 (基因名), Mediator of RNA polymerase II transcription subunit 31 (蛋白名), MED31_HUMAN.
产品名称:

Human MED31/ Mediator of RNA polymerase II transcription subunit 31 ELISA Kit
RNA聚合酶转录中介亚基31

货号:

E11531h

商标:
EIAab®
监管等级:
别名:

Mediator complex subunit 31, Mediator complex subunit SOH1, hSOH1, SOH1, CGI-125

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
1.56-100ng/mL
灵敏度:
0.39ng/ml
特异性:
Natural and recombinant human Mediator of RNA polymerase II transcription subunit 31
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
-
Human MED31 ELISA Kit
规格 & 价格: cart
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Human MED31 ELISA Kit
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产品说明书
说明书: 下载说明书
MSDS: MSDS


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤4.6%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤8.3%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

91

85-97

Plasma

93

87-99

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

104-114%

92-101%

89-89%

85-95%

EDTA plasma(n=5)

98-111%

107-116%

98-108%

93-102%

heparin plasma(n=5)

97-107%

 

109-119%

88-98%

88-97%

 

通用注释


亚单元:
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.


功能:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.


亚细胞位置:
Nucleus


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