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MED31 (GeneName), Mediator of RNA polymerase II transcription subunit 31 (ProteinName), MED31_HUMAN.
Product Name:

Human MED31/ Mediator of RNA polymerase II transcription subunit 31 ELISA Kit

Cat.#:

E11531h

Brand:
EIAab®
Regulatory Status:
Alternative:

Mediator complex subunit 31, Mediator complex subunit SOH1, hSOH1, SOH1, CGI-125

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100ng/mL
Sensitivity:
0.39ng/ml
Specificity:
Natural and recombinant human Mediator of RNA polymerase II transcription subunit 31
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human MED31 ELISA Kit
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Human MED31 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.3%

Recovery
Recovery was determined by spiking various levels of Mediator of RNA polymerase II transcription subunit 31 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

91

85-97

Plasma

93

87-99

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mediator of RNA polymerase II transcription subunit 31 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

104-114%

92-101%

89-89%

85-95%

EDTA plasma(n=5)

98-111%

107-116%

98-108%

93-102%

heparin plasma(n=5)

97-107%

 

109-119%

88-98%

88-97%

 

General Annotation


Sub Unit:
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.


Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.


Subcellular Location:
Nucleus


This product has not yet been referenced specifically in any publications.

[1].
"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."

[2].
"A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation."

[3].
"MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."

[4].
"Complete sequencing and characterization of 21,243 full-length human cDNAs."

[5].
"A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."

[6].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

[7].
"Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3."

[8].
"Architecture of the human interactome defines protein communities and disease networks."

[9].
"Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes."

[10].
"The BioPlex Network: A Systematic Exploration of the Human Interactome."
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