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Il1b (基因名), Interleukin-1 beta (蛋白名), IL1B_RAT.

产品名称:

Rat Il1b/ Interleukin-1 beta ELISA Kit
Interleukin-1β

货号:

E0563r

商标:

EIAab^®

监管等级:

RUO

别名:

IL-1 beta

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

78-5000pg/mL

灵敏度:

40.4pg/mL

特异性:

Natural and recombinant rat Interleukin-1 beta

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Neurosciences

规格 & 价格:

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Rat Il1b ELISA Kit

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产品说明书

数据表:

说明书:

MSDS:

在线询价

数据
文献 ( 10 )
序列 / 3D
常见问题解答
反馈墙 (0)

精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤4.2%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤6.9%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	91	85-97
Plasma	93	87-99

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	88-100%	88-100%	85-97%	89-99%
EDTA plasma(n=5)	102-112%	119-129%	109-117%	103-113%
heparin plasma(n=5)	81-90%	89-99%	111-119%	103-112%

通用注释

亚单元:

Monomer. Interacts with MEFV.

功能:

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.

亚细胞位置:

Cytoplasm Cytosol Lysosome Secreted Exosome Cytoplasmic vesicle Autophagosome Secreted The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood. On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B. 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes. However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes. 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis. 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome. These mechanisms may not be not mutually exclusive.

数据库链接

UniGene:

Rn.9869

SMR:

Q63264

STRING:

10116.ENSRNOP00000006308

Pfam:

PF00340

Uniprot:

Q63264

相关产品

Human IL1B ELISA Kit

Mouse Il1b ELISA Kit

Bovine IL1B ELISA Kit

Pig IL1B ELISA Kit

Dog IL1B ELISA Kit

序列长度:

268

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

268:

GNSNGRDIVD | FTMEPVSS

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"A biodegradable multifunctional porous microsphere composed of carrageenan for promoting imageable trans-arterial chemoembolization"

KL Liu, ZC Jin, XL Hu, et al.

From: International Journal of Biological Macromolecules

[2].

"Cynaroside protects the blue light-induced retinal degeneration through alleviating apoptosis and inducing autophagy in vitro and in vivo"

JH Feng, XW Dong, HL Yu, et al.

From: Phytomedicine

[3].

"Mast cell phenotypic plasticity and their activity under the influence of cathelicidin-related antimicrobial peptide (CRAMP) and IL-33 alarmins"

J Agier, E Brzezińska-Błaszczyk, S Różalska, et al.

From: Cellular Immunology

[4].

"Resistance exercise shifts the balance of renin-angiotensin system toward ACE2/Ang 1–7 axis and reduces inflammation in the kidney of diabetic rats"

R A Santos, V Guzzoni, K A S Silva, et al.

From: Life Sciences

[5].

"Different effectiveness of fungal pathogen-associated molecular patterns (PAMPs) in activating rat peritoneal mast cells"

P Żelechowska, E Brzezińska-Błaszczyk, J Agier, et al.

From: Immunology Letters

[6].

"Alleviating effects of morin against experimentally-induced diabetic osteopenia"

Hatem M Abuohashish,Salim S Al-Rejaie ,Khaled A Al-Hosaini,Mihir Y Parmar,Mohammed M Ahmed

From: Diabetology & Metabolic Syndrome

[7].

"Flavonoid, morin inhibits oxidative stress, inflammation and enhances neurotrophic support in the brain of streptozotocin-induced diabetic rats"

MS Ola, AM Aleisa, SS Al-Rejaie, et al.

From: Neurological Sciences

[8].

"Protective effects of rosuvastatin in a rat model of lung contusion: Stimulation of the cyclooxygenase 2–prostaglandin E-2 pathway"

O Dolkart, E Amar, S Shapira, et al.

From: Surgery

[9].

"Paeoniflorin ameliorates acute myocardial infarction of rats by inhibiting inflammation and inducible nitric oxide synthase signaling pathways"

Chen C, Du P, Wang J.

From: Molecular medicine reports

[10].

"Cynaroside protects the blue light-induced retinal degeneration through alleviating apoptosis and inducing autophagy in vitro and in vivo"

J-H Feng, X-W Dong, H-L Yu, et al.

From: Phytomedicine

[1].

大鼠Interleukin-1β(Il1b)ELISA试剂盒可以做多少个样本？

大鼠Interleukin-1β(Il1b)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

大鼠Interleukin-1β(Il1b)ELISA试剂盒使用视频？

大鼠Interleukin-1β(Il1b)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

大鼠Interleukin-1β(Il1b)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的大鼠Interleukin-1β(Il1b)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

大鼠Interleukin-1β(Il1b)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

大鼠Interleukin-1β(Il1b)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析大鼠Interleukin-1β(Il1b)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

大鼠Interleukin-1β(Il1b)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集大鼠Interleukin-1β(Il1b)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

大鼠Interleukin-1β(Il1b)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

大鼠Interleukin-1β(Il1b)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

大鼠Interleukin-1β(Il1b)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Rat Il1b/ Interleukin-1 beta ELISA Kit Interleukin-1β

E0563r

IL-1 beta

Rat Il1b/ Interleukin-1 beta ELISA Kit
Interleukin-1β