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IgG (Shorten Name),
Product Name:

Human IgG/ Immunoglobulin G ELISA Kit

 
Cat.#:

E0544h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
29.2 pg/mL
Specificity:
Natural and recombinant human Immunoglobulin G
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Immunology
Product Overview:
E0544h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Immunoglobulin G) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Immunoglobulin G. The ELISA analytical biochemical technique of the E0544h kit is based on Immunoglobulin G antibody-Immunoglobulin G antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Immunoglobulin G antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Immunoglobulin G. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human IgG ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.8%

Recovery
Recovery was determined by spiking various levels of Immunoglobulin G into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

95

89-101

Plasma

97

91-103

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin G and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

82-93%

101-111%

88-97%

96-104%

EDTA plasma(n=5)

105-115%

108-119%

107-117%

116-126%

heparin plasma(n=5)

105-114%

 

107-117%

107-117%

92-104%

 

General Annotation


Sub Unit:
N/A


Function:
IgG antibodies are involved in predominantly the secondary immune response. The presence of specific IgG, in general, corresponds to maturation of the antibody response.


Location:
Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains — two heavy chains γ and two light chains. Each IgG has two antigen binding sites. Other immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG constitutes 75% of serum immunoglobulins in humans. IgG molecules are synthesized and secreted by plasma B cells.


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