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Hif1a (基因名), Hypoxia-inducible factor 1-alpha (蛋白名), hif1a_mouse.
产品名称:

Mouse Hif1a/ Hypoxia-inducible factor 1-alpha ELISA Kit
缺氧诱导因子1-α

货号:

E0798m

商标:
EIAab®
监管等级:
别名:

ARNT-interacting protein, HIF-1-alpha

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
78-5000pg/mL
灵敏度:
39.8pg/mL
特异性:
Natural and recombinant mouse Hypoxia-inducible factor 1-alpha
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Cancer
Mouse Hif1a ELISA Kit
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Mouse Hif1a ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS
在线询价


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤7.1%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤9.1%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

98

92-104

Plasma

100

94-106

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

89-98%

95-104%

83-91%

109-118%

EDTA plasma(n=5)

107-116%

95-107%

106-117%

105-114%

heparin plasma(n=5)

100-112%

 

115-115%

105-105%

81-91%

 

通用注释


亚单元:
Interacts with HIF3A isoform 2 (PubMed:21546903). Interacts with the HIF1A beta/ARNT subunit; heterodimerization is required for DNA binding. Interacts with Interacts with NCOA1, NCOA2, APEX and HSP90. Interacts (hydroxylated within the ODD domain) with VHLL (via beta domain); the interaction, leads to polyubiquitination and subsequent HIF1A proteasomal degradation. During hypoxia, sumoylated HIF1A also binds VHL; the interaction promotes the ubiquitination of HIF1A. Interacts with SENP1; the interaction desumoylates HIF1A resulting in stabilization and activation of transcription. Interacts (Via the ODD domain) with ARD1A; the interaction appears not to acetylate HIF1A nor have any affect on protein stability, during hypoxia. Interacts with RWDD3; the interaction enhances HIF1A sumoylation. Interacts with RORA (via the DNA binding domain); the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. Interaction with PSMA7 inhibits the transactivation activity of HIF1A under both normoxic and hypoxia-mimicking conditions. Interacts with USP20. Interacts with RACK1; promotes HIF1A ubiquitination and proteasome-mediated degradation. Interacts with EP300 (via TAZ-type 1 domain); the interaction is stimulated in response to hypoxia and inhibited by CITED2. Interacts with CREBBP (via TAZ-type 1 domain). Interacts with SIRT2. Interacts (deacetylated form) with EGLN1 (By similarity). Interacts with TSGA10. Interacts (via N-terminus) with USP19. Interacts with COPS5; the interaction increases the transcriptional activity of HIF1A through increased stability. Interacts with CBFA2T3. Interacts with HSP90AA1 and HSP90AB1.


功能:
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.


亚细胞位置:
Cytoplasm Nucleus Nucleus speckle Colocalizes with HIF3A isoform 2 in the nucleus and speckles (PubMed:21546903). Cytoplasmic in normoxia, nuclear translocation in response to hypoxia (By similarity).


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