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THBS1 (基因名), Thrombospondin-1 (蛋白名), TSP1_HUMAN.
产品名称:

Human THBS1/ Thrombospondin-1 ELISA Kit
血小板反应蛋白1

货号:

E0611h

商标:
EIAab®
监管等级:
别名:

Glycoprotein G, TSP, TSP1

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.156-10ng/mL
灵敏度:
0.098ng/mL
特异性:
Natural and recombinant human Thrombospondin-1
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Cardiovascular
Human THBS1 ELISA Kit
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Human THBS1 ELISA Kit
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产品说明书
说明书: 下载说明书
MSDS: MSDS
在线询价


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤4.3%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤7.9%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

103

97-109

Plasma

105

99-111

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

97-107%

81-93%

92-105%

109-119%

EDTA plasma(n=5)

93-103%

86-99%

112-120%

103-115%

heparin plasma(n=5)

99-108%

 

82-92%

107-117%

99-109%

 

通用注释


亚单元:
Homotrimer; disulfide-linked. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS1 with CD36 (By similarity). Can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3. Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts with ATF6 (via lumenal domain) (By similarity).


功能:
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity).


亚细胞位置:
Secreted Cell surface Secreted Extracellular space Extracellular matrix Endoplasmic reticulum Sarcoplasmic reticulum Secreted by thrombin-activated platelets and binds to the cell surface in the presence of extracellular Ca(2+) (PubMed:6777381). Incorporated into the extracellular matrix of fibroblasts (PubMed:6341993). Also detected in the endoplasmic reticulum and sarcoplasmic reticulum where it plays a role in the ER stress response (By similarity).


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