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PPP1CA (基因名), Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (蛋白名), PP1A_HUMAN.

产品名称:

Human PPP1CA/ Serine/threonine-protein phosphatase PP1-alpha catalytic subunit ELISA Kit
丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基

货号:

E9568h

商标:

EIAab^®

监管等级:

RUO

别名:

PP-1A, PPP1A

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.312-20ng/mL

灵敏度:

0.15ng/mL

特异性:

Natural and recombinant human Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Cardiovascular

规格 & 价格:

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Human PPP1CA ELISA Kit

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产品说明书

数据表:

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤4.6%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤8.0%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	93	87-99
Plasma	95	89-101

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	94-104%	99-109%	93-93%	104-114%
EDTA plasma(n=5)	103-103%	84-94%	80-90%	106-115%
heparin plasma(n=5)	96-107%	95-105%	101-111%	101-111%

通用注释

亚单元:

PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A, PPP1R12B and PPP1R12C mediate binding to myosin. PPP1R3A (in skeletal muscle), PPP1R3B (in liver), PPP1R3C, PPP1R3D and PPP1R3F (in brain) mediate binding to glycogen. Interacts with PPP1R39 (By similarity). Interacts with BTBD10 (By similarity). Interacts with KCTD20 (By similarity). Interacts with PPP1R9A and PPP1R9B. Part of a complex containing PPP1R15B, PP1 and NCK1/2. Interacts with PHACTR4; which acts as an activator of PP1 activity (By similarity). Interacts with PPP1R15A and PPP1R15B; the interactions mediate binding to EIF2S1. Component of the MLL5-L complex, at least composed of KMT2E/MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with PPP1R7. Interacts with YLPM1. Forms a complex with ILF2, ILF3, YLPM1, KHDRBS1, RBMX and NCOA5. Interacts with NOM1 and PPP1R8. Interacts with HHV-1 ICP34.5. Interacts with PPP1R16B. Interacts with RPSA only in the presence of PPP1R16B. Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA or PPP1CB or PPP1CC. Interacts with PPP1R10/PNUTS and PPP1R8. Interacts with WDR82 in the presence of PPP1R10/PNUTS. Interacts with TRIM28; the interaction dephosphorylates TRIM28 on 'Ser-824' and forms a complex at the p21 promoter site. Interacts with isoform 1 and isoform 4 of NEK2. Interacts with FER; this promotes phosphorylation at Thr-320. Interacts with DAB2; the interaction is mutually exclusive with the AXIN1:PPP1CA interaction. Interacts with FOXP3 (PubMed:23396208). Interacts with CENPA (PubMed:25556658). Interacts with ATG16L1 (PubMed:26083323).

功能:

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Dephosphorylates CENPA (PubMed:25556658). Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy (PubMed:26083323).

亚细胞位置:

Cytoplasm Nucleus Nucleus Nucleoplasm Nucleus Nucleolus Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.

数据库链接

UniGene:

Hs.183994

SMR:

P62136

STRING:

9606.ENSP00000326031

KEGG:

hsa:5499

MIM:

176875

Pfam:

PF00149

Uniprot:

P62136

相关产品

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Rat Ppp1ca ELISA Kit

Bovine PPP1CA ELISA Kit

Dog PPP1CA ELISA Kit

Mouse Ppp1ca CLIA Kit

序列长度:

330

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

330:

KNKGKYGQFS | GLNPGGRPIT | PPRNSAKAKK |

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

人丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基(PPP1CA)ELISA试剂盒可以做多少个样本？

人丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基(PPP1CA)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基(PPP1CA)ELISA试剂盒使用视频？

人丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基(PPP1CA)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基(PPP1CA)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基(PPP1CA)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基(PPP1CA)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基(PPP1CA)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基(PPP1CA)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基(PPP1CA)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基(PPP1CA)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基(PPP1CA)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基(PPP1CA)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基(PPP1CA)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Human PPP1CA/ Serine/threonine-protein phosphatase PP1-alpha catalytic subunit ELISA Kit 丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基

E9568h

PP-1A, PPP1A

Human PPP1CA/ Serine/threonine-protein phosphatase PP1-alpha catalytic subunit ELISA Kit
丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基