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DDOST (基因名), Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit (蛋白名), OST48_HUMAN.
产品名称:

Human DDOST/ Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit ELISA Kit
多萜长醇二磷酸寡糖蛋白环糊精糖基转移

货号:

E2093h

商标:
EIAab®
监管等级:
别名:

OK/SW-cl.45, DDOST 48 kDa subunit, KIAA0115, OST48

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.312-20ng/mL
灵敏度:
0.12ng/mL
特异性:
Natural and recombinant human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
-
Human DDOST ELISA Kit
规格 & 价格: cart
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Human DDOST ELISA Kit
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产品说明书
说明书: 下载说明书
MSDS: MSDS
在线询价


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤4.6%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤7.3%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

98

92-104

Plasma

100

94-106

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

87-99%

96-106%

101-111%

87-97%

EDTA plasma(n=5)

92-104%

107-116%

91-103%

80-89%

heparin plasma(n=5)

110-120%

 

90-100%

110-118%

91-100%

 

通用注释


亚单元:
Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes.


功能:
Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Required for the assembly of both SST3A- and SS3B-containing OST complexes. Required for efficient N-glycosylation.


亚细胞位置:
Endoplasmic reticulum membrane Single-pass type I membrane protein


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