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PPP1R9B (基因名), Neurabin-2 (蛋白名), NEB2_HUMAN.

产品名称:

Human PPP1R9B/ Neurabin-2 ELISA Kit
Neurabin-2蛋白

货号:

E8951h

商标:

EIAab^®

监管等级:

RUO

别名:

Neurabin-II, Protein phosphatase 1 regulatory subunit 9B, Spinophilin, PPP1R6

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.156-10ng/mL

灵敏度:

0.098ng/mL

特异性:

Natural and recombinant human Neurabin-2

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Signal Transduction

规格 & 价格:

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Human PPP1R9B ELISA Kit

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤5.6%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤9.6%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	103	97-109
Plasma	105	99-111

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	99-109%	105-115%	111-121%	105-117%
EDTA plasma(n=5)	99-109%	105-115%	82-95%	96-105%
heparin plasma(n=5)	96-104%	101-111%	82-94%	98-108%

通用注释

亚单元:

Interacts with DCLK2 (By similarity). Possibly exists as a homodimer, homotrimer or a homotetramer. Interacts with F-actin, PPP1CA, neurabin-1, TGN38 and D(2) dopamine receptor. Interacts with RGS1, RGS2, RGS4, RGS19 and ADRA1B, ADRA2A, ADRA2B, ADRA2C, CDKN2A, PPP1R2, RASGFR1 and TIAM1. Interacts (via C-terminus) with SPATA13 (via C-terminal tail). Interacts with ADRA2B.

功能:

Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha-adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1 (By similarity). Required for hepatocyte growth factor (HGF)-induced cell migration.

亚细胞位置:

Cytoplasm Cytoskeleton Nucleus Cell projection Dendritic spine Cell junction Synapse Cell junction Adherens junction Cytoplasm Cell membrane Cell projection Lamellipodium Cell projection Filopodium Cell projection Ruffle membrane Enriched at synapse and cadherin-based cell-cell adhesion sites. In neurons, both cytosolic and membrane-associated, and highly enriched in the postsynaptic density apposed to exitatory synapses. Colocalizes with PPP1R2 at actin-rich adherens junctions in epithelial cells and in dendritic spines (By similarity). Accumulates in the lamellipodium, filopodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.

数据库链接

UniGene:

Hs.514323

SMR:

Q96SB3

KEGG:

hsa:84687

MIM:

603325

Pfam:

PF00595

Uniprot:

Q96SB3

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Rat Ppp1r9b CLIA Kit

Mouse Ppp1r9b Protein

序列长度:

815

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

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815:

ELEGNLQTLR | NSNST

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

人Neurabin-2蛋白(PPP1R9B)ELISA试剂盒可以做多少个样本？

人Neurabin-2蛋白(PPP1R9B)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人Neurabin-2蛋白(PPP1R9B)ELISA试剂盒使用视频？

人Neurabin-2蛋白(PPP1R9B)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人Neurabin-2蛋白(PPP1R9B)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人Neurabin-2蛋白(PPP1R9B)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人Neurabin-2蛋白(PPP1R9B)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人Neurabin-2蛋白(PPP1R9B)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人Neurabin-2蛋白(PPP1R9B)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人Neurabin-2蛋白(PPP1R9B)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人Neurabin-2蛋白(PPP1R9B)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人Neurabin-2蛋白(PPP1R9B)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人Neurabin-2蛋白(PPP1R9B)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人Neurabin-2蛋白(PPP1R9B)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Human PPP1R9B/ Neurabin-2 ELISA Kit Neurabin-2蛋白

E8951h

Neurabin-II, Protein phosphatase 1 regulatory subunit 9B, Spinophilin, PPP1R6

Human PPP1R9B/ Neurabin-2 ELISA Kit
Neurabin-2蛋白