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KIT (基因名), Mast/stem cell growth factor receptor Kit (蛋白名), KIT_PIG.

产品名称:

Pig KIT/ Mast/stem cell growth factor receptor Kit ELISA Kit
肥大/干细胞生长因子受体

货号:

E0121p

商标:

EIAab^®

监管等级:

RUO

别名:

Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, CD117, SCFR

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.312-20ng/mL

灵敏度:

0.15ng/mL

特异性:

Natural and recombinant pig Mast/stem cell growth factor receptor Kit

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

规格 & 价格:

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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通用注释

亚单元:

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC. Interacts with IL1RAP (independent of stimulation with KITLG/SCF). A mast cell-specific KITLG/SCF-induced interleukin-33 signaling complex contains IL1RL1, IL1RAP, KIT and MYD88.

功能:

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

亚细胞位置:

Cell membrane Single-pass type I membrane protein

数据库链接

UniGene:

Ssc.41222

SMR:

Q2HWD6

STRING:

9823.ENSSSCP00000009430

KEGG:

ssc:396810

Pfam:

PF00047

Uniprot:

Q2HWD6

相关产品

Human KIT ELISA Kit

Mouse Kit ELISA Kit

Bovine KIT ELISA Kit

Chicken KIT ELISA Kit

Dog KIT ELISA Kit

序列长度:

972

序列:

仅供参考，详细请咨询（services@eiaab.com）

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972:

VRINSVGSSA | SSTQPLLVHE | DV

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

猪肥大/干细胞生长因子受体(KIT)ELISA试剂盒可以做多少个样本？

猪肥大/干细胞生长因子受体(KIT)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

猪肥大/干细胞生长因子受体(KIT)ELISA试剂盒使用视频？

猪肥大/干细胞生长因子受体(KIT)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

猪肥大/干细胞生长因子受体(KIT)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的猪肥大/干细胞生长因子受体(KIT)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

猪肥大/干细胞生长因子受体(KIT)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

猪肥大/干细胞生长因子受体(KIT)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析猪肥大/干细胞生长因子受体(KIT)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

猪肥大/干细胞生长因子受体(KIT)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集猪肥大/干细胞生长因子受体(KIT)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

猪肥大/干细胞生长因子受体(KIT)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

猪肥大/干细胞生长因子受体(KIT)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

猪肥大/干细胞生长因子受体(KIT)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Pig KIT/ Mast/stem cell growth factor receptor Kit ELISA Kit 肥大/干细胞生长因子受体

E0121p

Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, CD117, SCFR

Pig KIT/ Mast/stem cell growth factor receptor Kit ELISA Kit
肥大/干细胞生长因子受体