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Il36b (基因名), Interleukin-36 beta (蛋白名), IL36B_MOUSE.
产品名称:

Mouse Il36b/ Interleukin-36 beta ELISA Kit
白介素36β

货号:

E2013m

商标:
EIAab®
监管等级:
别名:

Interleukin-1 family member 8, IL-1F8, Fil1e, Il1f8

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
78-5000 pg/mL
灵敏度:
39.4 pg/mL
特异性:
Natural and recombinant mouse Interleukin-36 beta
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
-
Mouse Il36b ELISA Kit
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Mouse Il36b ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS
在线询价


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: 0
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: 0
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

80

80-80

Plasma

80

80-80

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

96-107%

89-99%

91-100%

99-109%

EDTA plasma(n=5)

98-107%

107-116%

103-113%

95-104%

heparin plasma(n=5)

96-106%

 

107-115%

99-108%

86-95%

 

通用注释


亚单元:
N/A


功能:
Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensin 4 and beta-defensin 103 as well as a number of matrix metalloproteases (By similarity). Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. Induces the production of proinflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23, and activates p38 MAPK phosphorylation in BMDCs. Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by T-helper 1 (Th1) cells, cultured CD4(+) T-cells and splenocytes.


亚细胞位置:
Secreted Secreted upon LPS treatment followed by ATP-induced activation of P2rx7.


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