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IGF1R (基因名), Insulin-like growth factor 1 receptor (蛋白名), IGF1R_HUMAN.
产品名称:

Human IGF1R/ Insulin-like growth factor 1 receptor ELISA Kit
胰岛素样生长因子1受体

货号:

E1200h

商标:
EIAab®
监管等级:
别名:

Insulin-like growth factor I receptor, IGF-I receptor, CD221

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.312-20ng/mL
灵敏度:
0.098ng/mL
特异性:
Natural and recombinant human Insulin-like growth factor 1 receptor
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Cancer
Human IGF1R ELISA Kit
规格 & 价格: cart
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Human IGF1R ELISA Kit
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产品说明书
说明书: 下载说明书
MSDS: MSDS
在线询价


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤5.2%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤8.0%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

103

97-109

Plasma

105

99-111

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

105-115%

95-105%

103-115%

100-111%

EDTA plasma(n=5)

95-105%

105-115%

93-103%

110-119%

heparin plasma(n=5)

108-117%

 

99-111%

82-92%

105-115%

 

通用注释


亚单元:
Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Forms a hybrid receptor with INSR, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. Interacts with ARRB1 and ARRB2. Interacts with GRB10. Interacts with RACK1. Interacts with SOCS1, SOCS2 and SOCS3. Interacts with 14-3-3 proteins. Interacts with NMD2. Interacts with MAP3K5. Interacts with STAT3. Found in a ternary complex with IGF1 and ITGAV:ITGB3 or ITGA6:ITGB4 (PubMed:19578119, PubMed:22351760).


功能:
When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.


亚细胞位置:
Cell membrane Single-pass type I membrane protein


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