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DTNBP1 (基因名), Dysbindin (蛋白名), DTBP1_HUMAN.

产品名称:

Human DTNBP1/ Dysbindin ELISA Kit
?人小肌营养蛋白结合蛋白1

货号:

E14962h

商标:

EIAab^®

监管等级:

RUO

别名:

Biogenesis of lysosome-related organelles complex 1 subunit 8, Dysbindin-1, Dystrobrevin-binding protein 1, Hermansky-Pudlak syndrome 7 protein, BLOC-1 subunit 8, HPS7 protein, My031, BLOC1S8

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

78-5000pg/mL

灵敏度:

34pg/ml

特异性:

Natural and recombinant human Dysbindin

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

规格 & 价格:

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤5.7%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤7.5%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	98	92-104
Plasma	100	94-106

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	102-111%	86-95%	89-98%	108-116%
EDTA plasma(n=5)	103-115%	108-118%	104-115%	88-98%
heparin plasma(n=5)	100-112%	112-121%	103-113%	107-117%

通用注释

亚单元:

Interacts (via its coiled coil domain) with KXD1. Interacts with CMYA5, PI4K2 and RNF151 (By similarity). Component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1) composed of at least BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. Interacts directly in the complex with BLOC1S5, BLOC1S6 and SNAPIN/BLOC1S8. The BLOC-1 complex associates with the AP-3 protein complex and membrane protein cargos. This BLOC-1 complex also associates with the BLOC-2 complex in endosomes. Binds to DTNA and DTNB but may not be a physiological binding partner (PubMed:16980328). Interacts (isoform 1 and isoform 2 only) with the DNA-dependent protein kinase complex DNA-PK; the interaction phosphorylates DTNBP1 in vitro. Interacts directly in this complex with XRCC5 and XRCC6. Interacts with AP3M1, AP3B2 and TRIM32. Interacts with XPO1; the interaction exports DTNBP1 out of the nucleus.

功能:

Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Associates with the BLOC-2 complex to facilitate the transport of TYRP1 independent of AP-3 function. Plays a role in synaptic vesicle trafficking and in neurotransmitter release. Plays a role in the regulation of cell surface exposure of DRD2. May play a role in actin cytoskeleton reorganization and neurite outgrowth. May modulate MAPK8 phosphorylation. Appears to promote neuronal transmission and viability through regulating the expression of SNAP25 and SYN1, modulating PI3-kinase-Akt signaling and influencing glutamatergic release. Regulates the expression of SYN1 through binding to its promoter. Modulates prefrontal cortical activity via the dopamine/D2 pathway.

亚细胞位置:

Isoform 3 Cytoplasm Cytoplasmic vesicle membrane Peripheral membrane protein Cytoplasmic side Cytoplasmic vesicle Secretory vesicle Synaptic vesicle membrane Peripheral membrane protein Cytoplasmic side Endosome membrane Peripheral membrane protein Cytoplasmic side Melanosome membrane Peripheral membrane protein Cytoplasmic side Cell junction Synapse Postsynaptic cell membrane Endoplasmic reticulum Exclusivley cytoplasmic. Predominantly found in the postsynaptic density (PSD). Little association with synaptic vesicles. The BLOC-1 complex associates with the BLOC-2 complex in early endosome-associated tubules. Associated with the AP-3 complex at presynaptic terminals.

数据库链接

UniGene:

Hs.571148

SMR:

Q96EV8

STRING:

9606.ENSP00000341680

KEGG:

hsa:84062

MIM:

607145

Pfam:

PF04440

Uniprot:

Q96EV8

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Chicken DTNBP1 ELISA Kit

Mouse Dtnbp1 CLIA Kit

序列长度:

351

序列:

仅供参考，详细请咨询（services@eiaab.com）

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100:

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3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

人?人小肌营养蛋白结合蛋白1(DTNBP1)ELISA试剂盒可以做多少个样本？

人?人小肌营养蛋白结合蛋白1(DTNBP1)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人?人小肌营养蛋白结合蛋白1(DTNBP1)ELISA试剂盒使用视频？

人?人小肌营养蛋白结合蛋白1(DTNBP1)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人?人小肌营养蛋白结合蛋白1(DTNBP1)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人?人小肌营养蛋白结合蛋白1(DTNBP1)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人?人小肌营养蛋白结合蛋白1(DTNBP1)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人?人小肌营养蛋白结合蛋白1(DTNBP1)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人?人小肌营养蛋白结合蛋白1(DTNBP1)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人?人小肌营养蛋白结合蛋白1(DTNBP1)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人?人小肌营养蛋白结合蛋白1(DTNBP1)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人?人小肌营养蛋白结合蛋白1(DTNBP1)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人?人小肌营养蛋白结合蛋白1(DTNBP1)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人?人小肌营养蛋白结合蛋白1(DTNBP1)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Human DTNBP1/ Dysbindin ELISA Kit ?人小肌营养蛋白结合蛋白1

E14962h

Biogenesis of lysosome-related organelles complex 1 subunit 8, Dysbindin-1, Dystrobrevin-binding protein 1, Hermansky-Pudlak syndrome 7 protein, BLOC-1 subunit 8, HPS7 protein, My031, BLOC1S8

Human DTNBP1/ Dysbindin ELISA Kit
?人小肌营养蛋白结合蛋白1