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CASP14 (基因名), Caspase-14 (蛋白名), CASPE_HUMAN.
产品名称:

Human CASP14/ Caspase-14 ELISA Kit
半胱天冬酶14

货号:

E2284h

商标:
EIAab®
监管等级:
别名:

CASP-14

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.312-20ng/mL
灵敏度:
0.19ng/mL
特异性:
Natural and recombinant human Caspase-14
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Cancer
Human CASP14 ELISA Kit
规格 & 价格: cart
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Human CASP14 ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS
在线询价


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤4.1%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤7.5%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

98

92-104

Plasma

100

94-106

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

110-120%

92-104%

86-96%

102-112%

EDTA plasma(n=5)

105-114%

98-107%

98-108%

109-119%

heparin plasma(n=5)

108-116%

 

103-114%

99-109%

98-108%

 

通用注释


亚单元:
Heterodimer of a large and a small subunit, both processed from the precursor; the mature active form is a p17/p10 dimer and the intermediate form a p20/p8 dimer.


功能:
Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum (PubMed:15556625). Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin (By similarity). In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF (PubMed:16854378, PubMed:19960512). Involved in processing of prosaposin in the epidermis (By similarity). May be involved in retinal pigment epithelium cell barrier function (PubMed:25121097). Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD (PubMed:24743736).


亚细胞位置:
Cytoplasm Nucleus


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