首页 > 酶联免疫试剂盒 > Mouse Btk ELISA Kit

Btk (基因名), Tyrosine-protein kinase BTK (蛋白名), BTK_MOUSE.

产品名称:

Mouse Btk/ Tyrosine-protein kinase BTK ELISA Kit
BTK酪氨酸蛋白激

货号:

E1915m

商标:

EIAab^®

监管等级:

RUO

别名:

Agammaglobulinemia tyrosine kinase, B-cell progenitor kinase, Bruton tyrosine kinase, Kinase EMB, ATK, BPK, Bpk

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.156-10ng/mL

灵敏度:

0.092ng/mL

特异性:

Natural and recombinant mouse Tyrosine-protein kinase BTK

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Immunology

规格 & 价格:

关注

Mouse Btk ELISA Kit

邮箱 *

消息 *

Please 登录.

产品说明书

数据表:

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
反馈墙 (0)

精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤8.8%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤11.0%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-103
Plasma	99	93-105

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	87-97%	99-109%	103-112%	93-103%
EDTA plasma(n=5)	95-106%	84-97%	102-114%	94-103%
heparin plasma(n=5)	103-116%	97-107%	104-114%	103-113%

通用注释

亚单元:

Binds GTF2I through the PH domain. Interacts with SH3BP5 via the SH3 domain. Interacts with IBTK via its PH domain (By similarity). Interacts with ARID3A. Interacts with CAV1, FASLG, PIN1, TLR8 and TLR9.

功能:

Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.

亚细胞位置:

Cytoplasm Cell membrane Peripheral membrane protein Nucleus In steady state, BTK is predominantly cytosolic. Following B-cell receptor (BCR) engagement by antigen, translocates to the plasma membrane through its PH domain. Plasma membrane localization is a critical step in the activation of BTK. A fraction of BTK also shuttles between the nucleus and the cytoplasm, and nuclear export is mediated by the nuclear export receptor CRM1.

数据库链接

UniGene:

Mm.4475

SMR:

P35991

STRING:

10090.ENSMUSP00000033617

KEGG:

mmu:12229

Pfam:

PF00779

Uniprot:

P35991

相关产品

Human BTK ELISA Kit

Chicken BTK ELISA Kit

Human BTK CLIA Kit

Chicken BTK CLIA Kit

Human BTK Protein

序列长度:

659

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

659:

ILDVMDEES

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

小鼠BTK酪氨酸蛋白激(Btk)ELISA试剂盒可以做多少个样本？

小鼠BTK酪氨酸蛋白激(Btk)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

小鼠BTK酪氨酸蛋白激(Btk)ELISA试剂盒使用视频？

小鼠BTK酪氨酸蛋白激(Btk)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

小鼠BTK酪氨酸蛋白激(Btk)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的小鼠BTK酪氨酸蛋白激(Btk)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

小鼠BTK酪氨酸蛋白激(Btk)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

小鼠BTK酪氨酸蛋白激(Btk)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析小鼠BTK酪氨酸蛋白激(Btk)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

小鼠BTK酪氨酸蛋白激(Btk)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集小鼠BTK酪氨酸蛋白激(Btk)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

小鼠BTK酪氨酸蛋白激(Btk)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

小鼠BTK酪氨酸蛋白激(Btk)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

小鼠BTK酪氨酸蛋白激(Btk)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

反馈墙

评论数 : 0

所有用户

默认排序

Mouse Btk/ Tyrosine-protein kinase BTK ELISA Kit BTK酪氨酸蛋白激

E1915m

Agammaglobulinemia tyrosine kinase, B-cell progenitor kinase, Bruton tyrosine kinase, Kinase EMB, ATK, BPK, Bpk

Mouse Btk/ Tyrosine-protein kinase BTK ELISA Kit
BTK酪氨酸蛋白激