COL2A1 (基因名), Collagen alpha-1(II) chain (蛋白名), co2a1_human.
产品名称:
Human COL2A1/ Collagen alpha-1(II) chain Recombinant Protein
胶原蛋白alpha -(2)链
货号:
R0572h
商标:
EIAab®
监管等级:
别名:
Alpha-1 type II collagen
序列号:
P02458
来源:
E.coli
种属:
Human
标签:
His
序列:
23-268aa
预估分子量:
27.06 kDa (monomer)
纯度:
Greater than 95% by SDS-PAGE
浓度:
Reconstitution Dependent
形态:
Liquid
内毒素水平:
Please contact protein@eiaab.com The technician for more information.
应用:
存储缓冲液:
50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)
存储:
Store at -20°C. (Avoid repeated freezing and thawing.)
研究领域:
Signal Transduction
R&D 技术数据
The PCR product of human COL2A1 gene was determined by 1% Agarose stained with EB.
Recombinant human COL2A1 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.
基因位点
COL2A1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
通用注释
亚单元:
Homotrimers of alpha 1(II) chains.
功能:
Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
亚细胞位置:
Secreted
Extracellular space
Extracellular matrix
[1].
[1].
"A COL2A1 gene polymorphism is related with advanced stages of osteoarthritis of the knee in Mexican Mestizo population."
[2].
"COL1A1 and COL2A1 genes and myopia susceptibility: evidence of association and suggestive linkage to the COL2A1 locus."
[3].
"COL2A1 gene polymorphisms and susceptibility to osteoarthritis of the hand in Finnish women."
[4].
"Perthes' disease and the search for genetic associations: collagen mutations, Gaucher's disease and thrombophilia."
[5].
"Genetic and epigenetic factors at COL2A1 and ABCA4 influence clinical outcome in congenital toxoplasmosis."
[6].
"Missense and nonsense mutations in the alternatively-spliced exon 2 of COL2A1 cause the ocular variant of Stickler syndrome."
[7].
"A familial case of achondrogenesis type II caused by a dominant COL2A1 mutation and "patchy" expression in the mosaic father."
[8].
"Sex and ethnic differences in the association of ASPN, CALM1, COL2A1, COMP, and FRZB with genetic susceptibility to osteoarthritis of the knee."
[9].
"High efficiency of mutation detection in type 1 stickler syndrome using a two-stage approach: vitreoretinal assessment coupled with exon sequencing for screening COL2A1."
[10].
"A novel mutation of COL2A1 resulting in dominantly inherited rhegmatogenous retinal detachment."
[1].
人胶原蛋白alpha -(2)链(COL2A1)重组蛋白是否是无菌的?
蛋白试剂瓶和蛋白保存液是经过高压灭菌的,但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的,可以用0.2微米的滤器对蛋白进行过滤。
[2].
人胶原蛋白alpha -(2)链(COL2A1)重组蛋白的保存缓冲液是什么?
纯化后的蛋白保存在PBS(58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4)里,并往里面加入500mM咪唑和10%甘油。
[3].
怎样确定人胶原蛋白alpha -(2)链(COL2A1)重组蛋白的浓度?
蛋白浓度的确定没有一个统一的标准,这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。
[4].
人胶原蛋白alpha -(2)链(COL2A1)重组蛋白蛋白保存条件是怎样的?
蛋白应保存在 -20℃或 -80℃条件下,为了避免反复冻融,可以将蛋白分装成小份保存。
[5].
人胶原蛋白alpha -(2)链(COL2A1)重组蛋白是否可以用于活体实验?
重组蛋白没有用于任何的活体实验,因此蛋白的活性和半衰期是不确定的。
[6].
人胶原蛋白alpha -(2)链(COL2A1)重组蛋白的保质期是多久?
在适当的保存条件下,从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃,保证蛋白的保存浓度高于0.1mg/ml,限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题,请联系我们的技术支持。
[7].
你们蛋白和抗体的报价是怎么样的?
我们将根据你需要的蛋白和抗体的大小进行报价。
[8].
人胶原蛋白alpha -(2)链(COL2A1)重组蛋白是否能够提供蛋白片段?
我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分,我们将会按您的需求提供蛋白和抗体。
[9].
人胶原蛋白alpha -(2)链(COL2A1)重组蛋白的货期或发货时间一般是多长?
具体指标的货期需要确定。最快一周,最长可能一个月。
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