NAMPT (基因名), Nicotinamide phosphoribosyltransferase (蛋白名), nampt_human.
产品名称:
Human NAMPT/ Nicotinamide phosphoribosyltransferase Recombinant Protein
烟碱转磷酸核糖基酶/内脂素
货号:
R0638h
商标:
EIAab®
监管等级:
别名:
Pre-B-cell colony-enhancing factor 1, Visfatin, Pre-B cell-enhancing factor, NAmPRTase, PBEF, PBEF1
序列号:
P43490
来源:
E.coli
种属:
Human
标签:
His
序列:
18-174aa
预估分子量:
17.27 kDa (monomer)
纯度:
Greater than 95% by SDS-PAGE
浓度:
Reconstitution Dependent
形态:
Liquid
内毒素水平:
Please contact protein@eiaab.com The technician for more information.
应用:
存储缓冲液:
50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)
存储:
Store at -20°C. (Avoid repeated freezing and thawing.)
研究领域:
Immunology
R&D 技术数据
The PCR product of human NAMPT gene was determined by 1% Agarose stained with EB.
Recombinant human NAMPT protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.
基因位点
NAMPT Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
通用注释
亚单元:
Homodimer.
功能:
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).
亚细胞位置:
Nucleus
Cytoplasm
Secreted
Under non-inflammatory conditions, visfatin predominantly exhibits a granular pattern within the nucleus. Secreted by endothelial cells upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-fold higher concentrations compared to maternal serum.
[2].
Kazem Y M I, El Shebini S M, Moaty M I A, et al
[1].
"Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor."
[2].
"Genetic variant in visfatin gene promoter is associated with decreased risk of coronary artery disease in a Chinese population."
[3].
"The visfatin (PBEF1) G-948T gene polymorphism is associated with increased high-density lipoprotein cholesterol in obese subjects."
[4].
"Molecular basis for the inhibition of human NMPRTase, a novel target for anticancer agents."
[6].
"The concentrations of visfatin in the follicular fluids of women undergoing controlled ovarian stimulation are correlated to the number of oocytes retrieved."
[7].
"Dynamics of Nampt/visfatin and high molecular weight adiponectin in response to oral glucose load in obese and lean women."
[9].
"Visfatin, resistin, hsCRP and insulin resistance in relation to abdominal obesity in women with rheumatoid arthritis."
[10].
"Visfatin is not associated with inflammation or metabolic syndrome in patients with severe rheumatoid arthritis undergoing anti-TNF-alpha therapy."
[1].
人烟碱转磷酸核糖基酶/内脂素(NAMPT)重组蛋白是否是无菌的?
蛋白试剂瓶和蛋白保存液是经过高压灭菌的,但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的,可以用0.2微米的滤器对蛋白进行过滤。
[2].
人烟碱转磷酸核糖基酶/内脂素(NAMPT)重组蛋白的保存缓冲液是什么?
纯化后的蛋白保存在PBS(58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4)里,并往里面加入500mM咪唑和10%甘油。
[3].
怎样确定人烟碱转磷酸核糖基酶/内脂素(NAMPT)重组蛋白的浓度?
蛋白浓度的确定没有一个统一的标准,这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。
[4].
人烟碱转磷酸核糖基酶/内脂素(NAMPT)重组蛋白蛋白保存条件是怎样的?
蛋白应保存在 -20℃或 -80℃条件下,为了避免反复冻融,可以将蛋白分装成小份保存。
[5].
人烟碱转磷酸核糖基酶/内脂素(NAMPT)重组蛋白是否可以用于活体实验?
重组蛋白没有用于任何的活体实验,因此蛋白的活性和半衰期是不确定的。
[6].
人烟碱转磷酸核糖基酶/内脂素(NAMPT)重组蛋白的保质期是多久?
在适当的保存条件下,从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃,保证蛋白的保存浓度高于0.1mg/ml,限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题,请联系我们的技术支持。
[7].
你们蛋白和抗体的报价是怎么样的?
我们将根据你需要的蛋白和抗体的大小进行报价。
[8].
人烟碱转磷酸核糖基酶/内脂素(NAMPT)重组蛋白是否能够提供蛋白片段?
我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分,我们将会按您的需求提供蛋白和抗体。
[9].
人烟碱转磷酸核糖基酶/内脂素(NAMPT)重组蛋白的货期或发货时间一般是多长?
具体指标的货期需要确定。最快一周,最长可能一个月。
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